Overview
- External ontology
- mediadive.medium:J173 (source link unavailable) — BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=173 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn in favor of this source's bovine serum albumin input. Existing unrelated chemical annotations are preserved, not recertified. This repair does not assert equivalence with other BSK formulations or add organism-growth evidence.
Ingredients (0)
No ingredients recorded.
Preparation
- Adjust pH to 7.6 with 1 N NaOH at the source's pre-combination stage; a final combined-medium pH measurement is not stated.
- Sterilize by filtration through a 0.22 micrometer filter (Millipore), before the source's subsequent combination instruction.
- Autoclave Solution B separately. The JCM page's general default is 121 C for 15 min unless otherwise stated.
- Mix Solutions A and B, then add 40 ml heat-inactivated (56 C, 45 min) sterile rabbit serum (Pel-Freez Biologicals).
Solutions
Solution A
| Ingredient | Concentration | Notes |
|---|---|---|
| CMRL 1066 | 9.9 G_PER_L | MediaDive explicitly reports g_l=9.9 inside this stock preparation; source batch input is 4.95 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Sigma, with glutamate. |
| Bovine serum albumin | 50 G_PER_L | MediaDive explicitly reports g_l=50 inside this stock preparation; source batch input is 25 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Fraction V; Wako. |
| HEPES | 6 G_PER_L | MediaDive explicitly reports g_l=6 inside this stock preparation; source batch input is 3 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Sigma. |
| Trisodium citrate | 0.7 G_PER_L | MediaDive explicitly reports g_l=0.7 inside this stock preparation; source batch input is 0.35 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. |
| Glucose | 5 G_PER_L | MediaDive explicitly reports g_l=5 inside this stock preparation; source batch input is 2.5 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. |
| Sodium pyruvate | 0.8 G_PER_L | MediaDive explicitly reports g_l=0.8 inside this stock preparation; source batch input is 0.4 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Sigma. |
| N-Acetyl-D-glucosamine | 0.4 G_PER_L | MediaDive explicitly reports g_l=0.4 inside this stock preparation; source batch input is 0.2 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Sigma. |
| NaHCO3 | 2.2 G_PER_L | MediaDive explicitly reports g_l=2.2 inside this stock preparation; source batch input is 1.1 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: Sigma. |
| Yeast extract | 2 G_PER_L | MediaDive explicitly reports g_l=2 inside this stock preparation; source batch input is 1 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: BD-Difco, TC. |
| Distilled water | 0.5 L | Source water input 500 ml / 1000, in this preparation only. Not a measured final volume or a parent dose. |
Separate source preparation. Water input does not independently establish measured final stock volume or a fixed parent addition dose. MediaDive g_l values below are provider-reported stock rates, not final-medium concentrations.
Solution B
| Ingredient | Concentration | Notes |
|---|---|---|
| Neopeptone | 0.025 G_PER_L | MediaDive explicitly reports g_l=0.025 inside this stock preparation; source batch input is 2.5 mg. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. Source specification: BD-Difco. |
| Gelatin | 70 G_PER_L | MediaDive explicitly reports g_l=70 inside this stock preparation; source batch input is 7 g. Retain only at stock scope, not as an independently measured final volume or receiving-medium rate. |
| Distilled water | 0.1 L | Source water input 100 ml / 1000, in this preparation only. Not a measured final volume or a parent dose. |
Separate source preparation. Water input does not independently establish measured final stock volume or a fixed parent addition dose. MediaDive g_l values below are provider-reported stock rates, not final-medium concentrations.
NaOH solution (1 N)
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | Source titrant strength: 1 N. Normality is not silently converted to molarity; fixed addition dose and final-medium concentration are unspecified. |
Heat-inactivated sterile rabbit serum
Dose: 0.04 L
Pel-Freez Biologicals; heat-inactivated at 56 C for 45 min. Add 40 ml after combining Solutions A and B. This is a batch dose, not 40 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.878698Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J173 |
| 2026-03-13T06:19:14.011366Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002533 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.199657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BARBOUR-SFOENNER-KELLY (BSK) II MEDIUM" to "barbour_sfoenner_kelly_bsk_ii_medium" for consistent matching |
| 2026-06-05T07:40:50.494870+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T23:24:53.639909+00:00 | codex | RESTORED_SOURCE_SPECIFIC_BSK_FORMULATIONS_WITH_INGREDIENT_EVIDENCE | Restore source-specific albumin, quantity scopes, missing inputs and preparation boundaries. Preserve ID, prior history and unrelated groundings. Unknown doses, percentage bases and final volumes stay unknown; distinct formulations are not blended. |