Overview
- External ontology
- mediadive.medium:J181 (source link unavailable) — HORIKOSHI-I MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=181
Composition
flowchart LR
CultureMech_002542["horikoshi_i_medium"]
ing0_Glucose["Glucose"]
CultureMech_002542 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Polypeptone["Polypeptone"]
CultureMech_002542 --> ing1_Polypeptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002542 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_002542 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002542 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Agar["Agar"]
CultureMech_002542 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
11.1111 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Polypeptone | 5.55556 G_PER_L | ||
| 3 | Yeast extract | 5.55556 G_PER_L | ||
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.11111 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.222222 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | Agar | 16.6667 G_PER_L |
CHEBI:2509
agar
|
Preparation
- After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- horikoshi_i_medium (CultureMech:002542)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.894640Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J181 |
| 2026-03-13T06:19:14.049461Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002542 |
| 2026-03-17T06:30:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.242897+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HORIKOSHI-I MEDIUM" to "horikoshi_i_medium" for consistent matching |
| 2026-06-05T07:41:08.701174+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.815769+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.797269+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5627c0774ddc2893474534cae09e1bab60b3954fb272532928d7489e98542324 |