horikoshi_i_medium

Overview

External ontology

mediadive.medium:J181 (source link unavailable) — HORIKOSHI-I MEDIUM

Applications

• Microbial cultivation

pH

10.0

Notes

Source: JCM Medium 181 | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=181 JCM Medium 181 Horikoshi-I medium prepares a 900 mL agar base with glucose, Hipolypepton, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, and agar, then aseptically adds 100 mL sterile 10% Na2CO3 solution after autoclaving for a final pH of about 10.0.

Composition

flowchart LR
    CultureMech_002542["horikoshi_i_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_002542 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Hipolypepton__FUJIFILM_Wako_["Hipolypepton (FUJIFILM Wako)"]
    CultureMech_002542 --> ing1_Hipolypepton__FUJIFILM_Wako_
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002542 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002542 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002542 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Agar["Agar"]
    CultureMech_002542 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Distilled_water["Distilled water"]
    CultureMech_002542 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377

Ingredients (7)

Preparation

1. Prepare the Horikoshi-I agar base in 900 mL water.
2. Autoclave at 121 C for 15 min.
3. Aseptically add 100 mL of sterile 10% Na2CO3 solution.
4. Check the final pH to be about 10.0.

Solutions

Target organisms

No target organisms are recorded.

Provenance