Overview
- External ontology
- mediadive.medium:J206 (source link unavailable) — JCM MEDIUM No. 206
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=206
Composition
flowchart LR
CultureMech_002568["jcm_medium_no_206"]
ing0_Citric_acid["Citric acid"]
CultureMech_002568 --> ing0_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing0_Citric_acid -.-> CHEBI_30769
ing1_Tryptone["Tryptone"]
CultureMech_002568 --> ing1_Tryptone
ing2_Sulfur["Sulfur"]
CultureMech_002568 --> ing2_Sulfur
CHEBI_26833["CHEBI:26833"]
ing2_Sulfur -.-> CHEBI_26833
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002568 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_Resazurin["Resazurin"]
CultureMech_002568 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002568 --> ing5_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002568 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002568 --> ing7_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4_x_n_H2O -.-> CHEBI_86360
ing8_NaCl["NaCl"]
CultureMech_002568 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002568 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002568 --> ing10_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002568 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002568 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002568 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002568 --> ing14_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing14_AlK_SO4_2 -.-> CHEBI_86463
ing15_H3BO3["H3BO3"]
CultureMech_002568 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002568 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Citric acid
C6H8O7 192.123 g/mol
|
0.00495049 G_PER_L |
CHEBI:30769
citric acid
|
|
| 2 | Tryptone | 5.94059 G_PER_L | ||
| 3 |
Sulfur
S 32.067 g/mol
|
5.94059 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20).
- Use Salt base solution of Medium No. 151 with 17.0 g/L (final) NaCl and 2.5 mg/L (final) KI.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_206 (CultureMech:002568)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.958939Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J206 |
| 2026-03-13T06:19:14.212706Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002568 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.429638+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 206" to "jcm_medium_no_206" for consistent matching |
| 2026-06-05T07:41:09.914728+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.220978+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.077185+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 03e1bab02bacc134cb8c0dd564db1b4ed1e42ea473e4542feb8dc5c0e43c0c6c |