jcm_medium_no_206

CultureMech:002568 bacterial COMPLEX LIQUID
Original name: JCM MEDIUM No. 206

Overview

External ontology
mediadive.medium:J206 (source link unavailable) — JCM MEDIUM No. 206
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=206

Composition

flowchart LR
    CultureMech_002568["jcm_medium_no_206"]
    ing0_Citric_acid["Citric acid"]
    CultureMech_002568 --> ing0_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing0_Citric_acid -.-> CHEBI_30769
    ing1_Tryptone["Tryptone"]
    CultureMech_002568 --> ing1_Tryptone
    ing2_Sulfur["Sulfur"]
    CultureMech_002568 --> ing2_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing2_Sulfur -.-> CHEBI_26833
    ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002568 --> ing3_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_x_9_H2O -.-> CHEBI_76209
    ing4_Resazurin["Resazurin"]
    CultureMech_002568 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002568 --> ing5_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002568 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002568 --> ing7_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing7_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing8_NaCl["NaCl"]
    CultureMech_002568 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002568 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002568 --> ing10_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002568 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002568 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002568 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002568 --> ing14_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing14_AlK_SO4_2 -.-> CHEBI_86463
    ing15_H3BO3["H3BO3"]
    CultureMech_002568 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002568 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Citric acid
C6H8O7 192.123 g/mol
0.00495049 G_PER_L CHEBI:30769
citric acid
2 Tryptone 5.94059 G_PER_L
3 Sulfur
S 32.067 g/mol
5.94059 G_PER_L CHEBI:26833
sulfur atom
4 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49505 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
5 Resazurin
C12H7NO4 229.191 g/mol
0.000990099 G_PER_L CHEBI:8806
Resazurin
6 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
9 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
15 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
16 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20).
  2. Use Salt base solution of Medium No. 151 with 17.0 g/L (final) NaCl and 2.5 mg/L (final) KI.
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.958939Z mediadive-import Imported from MediaDive Source: JCM, ID: J206
2026-03-13T06:19:14.212706Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002568
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.429638+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 206" to "jcm_medium_no_206" for consistent matching
2026-06-05T07:41:09.914728+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.220978+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.