Overview
- External ontology
- mediadive.medium:J220 (source link unavailable) — SC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=220 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002582["sc_medium"]
ing0_Corn_meal_agar["Corn meal agar"]
CultureMech_002582 --> ing0_Corn_meal_agar
CHEBI_2509["CHEBI:2509"]
ing0_Corn_meal_agar -.-> CHEBI_2509
ing1_Phytone_peptone["Phytone peptone"]
CultureMech_002582 --> ing1_Phytone_peptone
ing2_K2HPO4["K2HPO4"]
CultureMech_002582 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_002582 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002582 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Hemin["Hemin"]
CultureMech_002582 --> ing5_Hemin
CHEBI_50385["CHEBI:50385"]
ing5_Hemin -.-> CHEBI_50385
ing6_Tryptone["Tryptone"]
CultureMech_002582 --> ing6_Tryptone
ing7_Yeast_extract["Yeast extract"]
CultureMech_002582 --> ing7_Yeast_extract
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_002582 --> ing8_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Glucose["Glucose"]
CultureMech_002582 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Cysteine["Cysteine"]
CultureMech_002582 --> ing10_Cysteine
CHEBI_15356["CHEBI:15356"]
ing10_Cysteine -.-> CHEBI_15356
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Corn meal agar | 16.4093 G_PER_L |
CHEBI:2509
agar
|
|
| 2 | Phytone peptone | 7.72201 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.965251 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.965251 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.19305 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
15 G_PER_L |
CHEBI:50385
hemin
|
|
| 7 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 8 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 11 |
Cysteine
C3H7NO2S 121.161 g/mol
|
10 G_PER_L |
CHEBI:15356
cysteine
|
Preparation
- Adjust pH to 6.6.
- Separately sterilize bovine serum albumin, glucose and cysteine solutions by filtration, and aseptically add to the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J220_SC_MEDIUM (CultureMech:002582)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.997461Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J220 |
| 2026-03-13T06:19:14.313039Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002582 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.542392+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SC MEDIUM" to "sc_medium" for consistent matching |
| 2026-06-05T07:40:16.862479+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.844430+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.826668+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3d6f0fb48f0ae595982fbd19697ef073edfea24b19e4364c69f4c24798115161 |