Overview
- External ontology
- mediadive.medium:J220 (source link unavailable) — SC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=220 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact source specifies albumin fraction V, not LB broth. Remove only the reviewed LB supplier nodes and restore the source material at its preparation stage without inventing chemical grounding.
Composition
flowchart LR
CultureMech_002582["sc_medium"]
ing0_Corn_meal_agar["Corn meal agar"]
CultureMech_002582 --> ing0_Corn_meal_agar
CHEBI_2509["CHEBI:2509"]
ing0_Corn_meal_agar -.-> CHEBI_2509
ing1_Phytone_peptone["Phytone peptone"]
CultureMech_002582 --> ing1_Phytone_peptone
ing2_K2HPO4["K2HPO4"]
CultureMech_002582 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_002582 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002582 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Distilled_water["Distilled water"]
CultureMech_002582 --> ing5_Distilled_water
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Corn meal agar |
CHEBI:2509
agar
|
Source batch input: 17 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco. | |
| 2 | Phytone peptone | Source batch input: 8 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-BBL. | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 0.2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 6 | Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. |
Preparation
- Adjust pH to 6.6.
- Separately sterilize bovine serum albumin, glucose and cysteine solutions by filtration, and aseptically add to the medium.
Solutions
Hemin solution (0.1% in 0.05 N NaOH)
Dose: 0.015 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Hemin | Source reagent label: 0.1% in 0.05 N NaOH. Percentage basis is unspecified; no numeric stock strength or stock-batch mass is asserted. The enclosing descriptor holds the receiving-batch volume. |
Source preparation input: 15 ml = 0.015 L per receiving batch, not grams per liter or a dose per final liter. Source reagent label: 0.1% in 0.05 N NaOH; percentage basis and stock preparation amounts are unspecified.
NaOH solution (0.05 N)
| Ingredient | Concentration | Notes |
|---|---|---|
| NaOH | Source hemin-stock solvent: 0.05 N NaOH. Its addition volume and stock preparation amounts are unspecified. Normality is not converted to molarity and is not a final-medium concentration. |
Source hemin-stock solvent: 0.05 N NaOH. Its addition volume and stock preparation amounts are unspecified. Normality is not converted to molarity and is not a final-medium concentration.
20% bovine serum albumin fraction V solution
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Bovine serum albumin | Source reagent label: 20%. Percentage basis is unspecified; no numeric stock strength or stock-batch mass is asserted. The enclosing descriptor holds the receiving-batch volume. Source material qualifier: fraction V. |
Source preparation input: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter. Source reagent label: 20%; percentage basis and stock preparation amounts are unspecified. Source material qualifier: fraction V.
50% glucose solution
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Glucose | Source reagent label: 50%. Percentage basis is unspecified; no numeric stock strength or stock-batch mass is asserted. The enclosing descriptor holds the receiving-batch volume. |
Source preparation input: 1 ml = 0.001 L per receiving batch, not grams per liter or a dose per final liter. Source reagent label: 50%; percentage basis and stock preparation amounts are unspecified.
10% cysteine solution
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Cysteine | Source reagent label: 10%. Percentage basis is unspecified; no numeric stock strength or stock-batch mass is asserted. The enclosing descriptor holds the receiving-batch volume. |
Source preparation input: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter. Source reagent label: 10%; percentage basis and stock preparation amounts are unspecified.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.997461Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J220 |
| 2026-03-13T06:19:14.313039Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002582 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.542392+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SC MEDIUM" to "sc_medium" for consistent matching |
| 2026-06-05T07:40:16.862479+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.844430+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T21:15:01.038694+00:00 | codex | RESTORED_JCM_ALBUMIN_IDENTITIES_AND_REAGENT_INPUTS_WITH_EVIDENCE | Removed false LB expansions and restored albumin fraction V, source batch inputs, reagent doses and late preparations with exact evidence. Unknown percentage bases, final volumes, stock strengths and chemical groundings remain unresolved. The JCM201 original-source access gap is disclosed, not treated as corroboration. |