Overview
- External ontology
- mediadive.medium:J231 (source link unavailable) — METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=231
Composition
flowchart LR
CultureMech_002592["methanobacterium_thermoautotrophicum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002592 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002592 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_NaCl["NaCl"]
CultureMech_002592 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002592 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002592 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002592 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_K2HPO4["K2HPO4"]
CultureMech_002592 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Na2CO3["Na2CO3"]
CultureMech_002592 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002592 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002592 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Resazurin["Resazurin"]
CultureMech_002592 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Na2_EDTA["Na2-EDTA"]
CultureMech_002592 --> ing11_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing11_Na2_EDTA -.-> CHEBI_64734
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002592 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002592 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002592 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002592 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002592 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_H3BO3["H3BO3"]
CultureMech_002592 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002592 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002592 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Biotin["Biotin"]
CultureMech_002592 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_002592 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002592 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002592 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_002592 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002592 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002592 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_002592 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002592 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_002592 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1.6 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.6, 1.0] |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.32 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.12, 6.2] |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.21000000000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.08, 0.13] |
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10400000000000001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.004, 0.1] |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.15 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.64 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.55 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.018 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.011 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except cysteine x HCl and Na2S x 9H2O, and adjust pH to 7.2. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20). Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 100 kPa H2-CO2 (80:20).
- Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanobacterium_thermoautotrophicum_medium (CultureMech:002592)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.031440Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J231 |
| 2026-03-13T03:08:54.955674Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.395566Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002592 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.641405+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM" to "methanobacterium_thermoautotrophicum_medium" for consistent matching |
| 2026-06-05T07:41:30.836750+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.710469+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:29.645641+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bf0d1fab41c612f2b213b50832ba589967df15d54562a660e2c67d6c42e11525 |