methanobacterium_thermoautotrophicum_medium

CultureMech:002592 archaea DEFINED LIQUID
Original name: METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM

Overview

External ontology
mediadive.medium:J231 (source link unavailable) — METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=231

Composition

flowchart LR
    CultureMech_002592["methanobacterium_thermoautotrophicum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002592 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002592 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_NaCl["NaCl"]
    CultureMech_002592 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002592 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002592 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002592 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002592 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Na2CO3["Na2CO3"]
    CultureMech_002592 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002592 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002592 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Resazurin["Resazurin"]
    CultureMech_002592 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2_EDTA["Na2-EDTA"]
    CultureMech_002592 --> ing11_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing11_Na2_EDTA -.-> CHEBI_64734
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002592 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002592 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002592 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002592 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002592 --> ing16_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing17_H3BO3["H3BO3"]
    CultureMech_002592 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002592 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002592 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Biotin["Biotin"]
    CultureMech_002592 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_002592 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002592 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002592 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_002592 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002592 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002592 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_002592 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002592 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_002592 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.5 G_PER_L CHEBI:62946
ammonium sulfate
3 NaCl
Cl.Na 58.443 g/mol
1.6 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.6, 1.0]
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.32 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.12, 6.2]
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.21000000000000002 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.08, 0.13]
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.10400000000000001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.004, 0.1]
7 K2HPO4
HO4P.2K 174.174 g/mol
0.15 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Na2CO3
CO3.2Na 105.988 g/mol
4 G_PER_L CHEBI:29377
sodium carbonate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
11 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
12 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.64 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.55 G_PER_L CHEBI:86360
manganese(II) sulfate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.17 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.05 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.018 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.011 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
21 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
22 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
23 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
28 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
29 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
30 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients, except cysteine x HCl and Na2S x 9H2O, and adjust pH to 7.2. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20). Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 100 kPa H2-CO2 (80:20).
  2. Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.031440Z mediadive-import Imported from MediaDive Source: JCM, ID: J231
2026-03-13T03:08:54.955674Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:14.395566Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002592
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.641405+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM" to "methanobacterium_thermoautotrophicum_medium" for consistent matching
2026-06-05T07:41:30.836750+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.710469+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.