Overview
- External ontology
- mediadive.medium:J238 (source link unavailable) — ARTIFICIAL SOIL AGAR
- Applications
-
- Microbial cultivation
- pH
- 6.7
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=238
Composition
flowchart LR
CultureMech_002598["artificial_soil_agar"]
ing0_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_002598 --> ing0_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing0_CaSO4_x_2_H2O -.-> CHEBI_32583
ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_002598 --> ing1_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002598 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_K2SO4["K2SO4"]
CultureMech_002598 --> ing3_K2SO4
CHEBI_32036["CHEBI:32036"]
ing3_K2SO4 -.-> CHEBI_32036
ing4_K2HPO4["K2HPO4"]
CultureMech_002598 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_NaHCO3["NaHCO3"]
CultureMech_002598 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002598 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_Yeast_extract["Yeast extract"]
CultureMech_002598 --> ing7_Yeast_extract
ing8_Glucose["Glucose"]
CultureMech_002598 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Soil_extract["Soil extract"]
CultureMech_002598 --> ing9_Soil_extract
ing10_Agar["Agar"]
CultureMech_002598 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
1.01 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 2 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.49 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
K2SO4
2K.O4S 174.259 g/mol
|
0.025 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.005 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.001 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 8 | Yeast extract | 0.1 G_PER_L | ||
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
0.01 G_PER_L |
CHEBI:17234
glucose
|
|
| 10 | Soil extract | 50 G_PER_L | ||
| 11 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 6.6-6.8.
- To prepare soil extract, equal volumes of leafy soil and tap water are boiled for 2 hr and then cleared by centrifugation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- artificial_soil_agar (CultureMech:002598)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.054300Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J238 |
| 2026-03-13T06:19:14.450157Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002598 |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.718476+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARTIFICIAL SOIL AGAR" to "artificial_soil_agar" for consistent matching |
| 2026-06-05T07:40:49.823906+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.569953+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.106721+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 25f1582ee8009b051affe23ec612682d0ca189180d96df62d8d50210b6c22688 |