artificial_soil_agar

CultureMech:002598 bacterial COMPLEX SOLID_AGAR
Original name: ARTIFICIAL SOIL AGAR

Overview

External ontology
mediadive.medium:J238 (source link unavailable) — ARTIFICIAL SOIL AGAR
Applications
  • Microbial cultivation
pH
6.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=238

Composition

flowchart LR
    CultureMech_002598["artificial_soil_agar"]
    ing0_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002598 --> ing0_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing0_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_002598 --> ing1_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002598 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2SO4["K2SO4"]
    CultureMech_002598 --> ing3_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing3_K2SO4 -.-> CHEBI_32036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002598 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_NaHCO3["NaHCO3"]
    CultureMech_002598 --> ing5_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing5_NaHCO3 -.-> CHEBI_32139
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002598 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002598 --> ing7_Yeast_extract
    ing8_Glucose["Glucose"]
    CultureMech_002598 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Soil_extract["Soil extract"]
    CultureMech_002598 --> ing9_Soil_extract
    ing10_Agar["Agar"]
    CultureMech_002598 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
1.01 G_PER_L CHEBI:32583
calcium sulfate dihydrate
2 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.49 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 K2SO4
2K.O4S 174.259 g/mol
0.025 G_PER_L CHEBI:32036
potassium sulfate
5 K2HPO4
HO4P.2K 174.174 g/mol
0.005 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 NaHCO3
CHO3.Na 84.006 g/mol
0.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.001 G_PER_L CHEBI:86254
iron trichloride hexahydrate
8 Yeast extract 0.1 G_PER_L
9 Glucose
C6H12O6 180.156 g/mol
0.01 G_PER_L CHEBI:17234
glucose
10 Soil extract 50 G_PER_L
11 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 6.6-6.8.
  2. To prepare soil extract, equal volumes of leafy soil and tap water are boiled for 2 hr and then cleared by centrifugation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.054300Z mediadive-import Imported from MediaDive Source: JCM, ID: J238
2026-03-13T06:19:14.450157Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002598
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.718476+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ARTIFICIAL SOIL AGAR" to "artificial_soil_agar" for consistent matching
2026-06-05T07:40:49.823906+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.569953+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.