Overview
- External ontology
- mediadive.medium:J239 (source link unavailable) — JCM MEDIUM No. 239
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=239
Composition
flowchart LR
CultureMech_002599["jcm_medium_no_239"]
ing0_L_Asparagine["L-Asparagine"]
CultureMech_002599 --> ing0_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing0_L_Asparagine -.-> CHEBI_17196
ing1_Glycerol["Glycerol"]
CultureMech_002599 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_K2HPO4["K2HPO4"]
CultureMech_002599 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Polypeptone["Polypeptone"]
CultureMech_002599 --> ing3_Polypeptone
ing4_Agar["Agar"]
CultureMech_002599 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002599 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002599 --> ing6_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002599 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_Thiamine_HCl["Thiamine HCl"]
CultureMech_002599 --> ing8_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing8_Thiamine_HCl -.-> CHEBI_49105
ing9_Riboflavin["Riboflavin"]
CultureMech_002599 --> ing9_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing9_Riboflavin -.-> CHEBI_17015
ing10_Nicotinate["Nicotinate"]
CultureMech_002599 --> ing10_Nicotinate
CHEBI_15940["CHEBI:15940"]
ing10_Nicotinate -.-> CHEBI_15940
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002599 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Myo_inositol["Myo-inositol"]
CultureMech_002599 --> ing12_Myo_inositol
CHEBI_17268["CHEBI:17268"]
ing12_Myo_inositol -.-> CHEBI_17268
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002599 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002599 --> ing14_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
ing15_D_Biotin["D-Biotin"]
CultureMech_002599 --> ing15_D_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_D_Biotin -.-> CHEBI_15956
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
90.9091 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
181.818 G_PER_L |
CHEBI:17754
glycerol
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
90.9091 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 | Polypeptone | 2727.27 G_PER_L | ||
| 5 | Agar | 1363.64 G_PER_L |
CHEBI:2509
agar
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 10 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 11 |
Nicotinate
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.05 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Myo-inositol
C6H12O6 180.156 g/mol
|
0.05 G_PER_L |
CHEBI:17268
myo-inositol
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 16 |
D-Biotin
C10H16N2O3S 244.316 g/mol
|
0.025 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_239 (CultureMech:002599)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.056821Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J239 |
| 2026-02-06T05:10:29.394524Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:14.457377Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002599 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.726313+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 239" to "jcm_medium_no_239" for consistent matching |
| 2026-06-05T07:41:09.979424+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.244325+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.173377+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b6cfb07efdb59904b685989d68967fc98ab0be3ef3dd2abba4fc244ca915ad4a |