jcm_medium_no_239

CultureMech:002599 bacterial COMPLEX SOLID_AGAR
Original name: JCM MEDIUM No. 239

Overview

External ontology
mediadive.medium:J239 (source link unavailable) — JCM MEDIUM No. 239
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=239

Composition

flowchart LR
    CultureMech_002599["jcm_medium_no_239"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_002599 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Glycerol["Glycerol"]
    CultureMech_002599 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002599 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Polypeptone["Polypeptone"]
    CultureMech_002599 --> ing3_Polypeptone
    ing4_Agar["Agar"]
    CultureMech_002599 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002599 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Riboflavin["Riboflavin"]
    CultureMech_002599 --> ing6_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing6_Riboflavin -.-> CHEBI_17015
    ing7_Nicotinate["Nicotinate"]
    CultureMech_002599 --> ing7_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing7_Nicotinate -.-> CHEBI_15940
    ing8_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002599 --> ing8_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing8_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing9_Myo_inositol["Myo-inositol"]
    CultureMech_002599 --> ing9_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing9_Myo_inositol -.-> CHEBI_17268
    ing10_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002599 --> ing10_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing10_Calcium_pantothenate -.-> CHEBI_31345
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002599 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_D_Biotin["D-Biotin"]
    CultureMech_002599 --> ing12_D_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_D_Biotin -.-> CHEBI_15956

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 L-Asparagine
C4H8N2O3 132.119 g/mol
90.9091 G_PER_L CHEBI:17196
L-asparagine
2 Glycerol
C3H8O3 92.094 g/mol
181.818 G_PER_L CHEBI:17754
glycerol
3 K2HPO4
HO4P.2K 174.174 g/mol
90.9091 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Polypeptone 2727.27 G_PER_L
5 Agar 1363.64 G_PER_L CHEBI:2509
agar
6 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
7 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
8 Nicotinate
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
9 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.05 G_PER_L CHEBI:30961
pyridoxine hydrochloride
10 Myo-inositol
C6H12O6 180.156 g/mol
0.05 G_PER_L CHEBI:17268
myo-inositol
11 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
12 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
13 D-Biotin
C10H16N2O3S 244.316 g/mol
0.025 G_PER_L CHEBI:15956
biotin

Preparation

  1. Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.

Solutions

Trace salt solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.056821Z mediadive-import Imported from MediaDive Source: JCM, ID: J239
2026-02-06T05:10:29.394524Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:14.457377Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002599
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.726313+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 239" to "jcm_medium_no_239" for consistent matching
2026-06-05T07:41:09.979424+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.244325+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T07:04:21.847651+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).