sulfophobococcus_medium

Overview

External ontology

mediadive.medium:J252 (source link unavailable) — SULFOPHOBOCOCCUS MEDIUM

Applications

• Microbial cultivation

pH

7.6

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=252 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_002612["sulfophobococcus_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002612 --> ing0_Yeast_extract
    ing1_Tryptone["Tryptone"]
    CultureMech_002612 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002612 --> ing2_Yeast_extract
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_002612 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002612 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_002612 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002612 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002612 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_KCl["KCl"]
    CultureMech_002612 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_ZnCl2["ZnCl2"]
    CultureMech_002612 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002612 --> ing10_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002612 --> ing11_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing12_Glycine["Glycine"]
    CultureMech_002612 --> ing12_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing12_Glycine -.-> CHEBI_15428
    ing13_Na2CO3["Na2CO3"]
    CultureMech_002612 --> ing13_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing13_Na2CO3 -.-> CHEBI_29377
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002612 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Resazurin["Resazurin"]
    CultureMech_002612 --> ing15_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing15_Resazurin -.-> CHEBI_8806

Ingredients (16)

Preparation

1. Mix components except yeast extract, bovine serum albumin and Na2S x 9H2O. Bring to a boil for 5-10 sec and flush with N2. Adjust pH to 7.6 at 80-90C. After cooling to room temperature, dispense the medium into serum bottles under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave yeast extract as a 10% solution and neutralized Na2S x 9H2O as a 5% solution under a N2 atmosphere. Filter-sterilize bovine serum albumin as a 10% (w/v) solution under a N2 atmosphere. Before inoculation, aseptically and anaerobically add the yeast extract, bovine serum albumin and neutralized Na2S x 9H2O solutions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• sulfophobococcus_medium (CultureMech:002612)