Overview
- External ontology
- mediadive.medium:J252 (source link unavailable) — SULFOPHOBOCOCCUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=252 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The exact recipe specifies albumin, not LB broth. Remove only the three reviewed LB supplier nodes and restore albumin at its source-defined preparation stage. Genuine yeast extract and unrelated annotations are preserved.
Composition
flowchart LR
CultureMech_002612["sulfophobococcus_medium"]
ing0_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002612 --> ing0_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing0_FeSO4_x_7_H2O -.-> CHEBI_75836
ing1_Na2_EDTA["Na2-EDTA"]
CultureMech_002612 --> ing1_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing1_Na2_EDTA -.-> CHEBI_64734
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002612 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002612 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KCl["KCl"]
CultureMech_002612 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_ZnCl2["ZnCl2"]
CultureMech_002612 --> ing5_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing5_ZnCl2 -.-> CHEBI_49976
ing6_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002612 --> ing6_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing6_MnSO4_x_n_H2O -.-> CHEBI_86360
ing7_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002612 --> ing7_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing7_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing8_Glycine["Glycine"]
CultureMech_002612 --> ing8_Glycine
CHEBI_15428["CHEBI:15428"]
ing8_Glycine -.-> CHEBI_15428
ing9_Na2CO3["Na2CO3"]
CultureMech_002612 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Resazurin["Resazurin"]
CultureMech_002612 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Distilled_water["Distilled water"]
CultureMech_002612 --> ing11_Distilled_water
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Source batch input: 50 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 2 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Source batch input: 32 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
Source batch input: 66 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 31 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 5 |
KCl
Cl.K 74.551 g/mol
|
CHEBI:32588
potassium chloride
|
Source batch input: 31 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 6 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
CHEBI:49976
zinc dichloride
|
Source batch input: 2.1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 7 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
CHEBI:86360
manganese(II) sulfate
|
Source batch input: 2.3 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 8 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
CHEBI:131366
disodium tetraborate decahydrate
|
Source batch input: 1.8 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 9 |
Glycine
C2H5NO2 75.067 g/mol
|
CHEBI:15428
glycine
|
Source batch input: 1.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
CHEBI:29377
sodium carbonate
|
Source batch input: 230 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 12 | Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. |
Preparation
- Mix components except yeast extract, bovine serum albumin and Na2S·9H2O.
- Bring to a boil for 5-10 sec and flush with N2.
- Adjust pH to 7.6 at 80-90C.
- After cooling to room temperature, dispense the medium into serum bottles under a N2 atmosphere, seal with butyl rubber stoppers and autoclave.
- Separately autoclave yeast extract as a 10% solution and neutralized Na2S·9H2O as a 5% solution under a N2 atmosphere.
- Filter-sterilize bovine serum albumin as a 10% (w/v) solution under a N2 atmosphere.
- Before inoculation, aseptically and anaerobically add the yeast extract, bovine serum albumin and neutralized Na2S·9H2O solutions.
Solutions
10% yeast extract solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. Source qualifier: BD-Difco. |
Separate stock preparation added aseptically and anaerobically under N2 before inoculation. The source table mass is its contribution to the receiving batch, not a mass for preparing an unspecified stock volume. The stock percentage basis and fixed dose are unspecified.
10% (w/v) bovine serum albumin solution
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Bovine serum albumin | 10 PERCENT_W_V | Source batch input: 1 g. Final preparation volume is not independently established; the source batch amount is retained without asserting a final concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. Explicit albumin-stock strength is 10% (w/v) = 10 g per 100 ml = 100 g/L. This is not the receiving-medium concentration. |
Separate stock preparation added aseptically and anaerobically under N2 before inoculation. The source table mass is its contribution to the receiving batch, not a mass for preparing an unspecified stock volume.
Derived receiving-batch dose: source contribution 1 g / explicit stock strength (10 g / 0.1 L = 100 g/L) = 0.01 L (10 ml). Both inputs describe this recipe's separately filtered albumin stock. No final-medium volume is needed or inferred; the dose is calculated, not directly printed in the source.
5% neutralized sodium sulfide nonahydrate solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9 H2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
Separate stock preparation added aseptically and anaerobically under N2 before inoculation. The source table mass is its contribution to the receiving batch, not a mass for preparing an unspecified stock volume. The stock percentage basis and fixed dose are unspecified.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.093680Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J252 |
| 2026-03-13T06:19:14.547484Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002612 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.825693+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOPHOBOCOCCUS MEDIUM" to "sulfophobococcus_medium" for consistent matching |
| 2026-06-05T07:41:47.515753+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.047386+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T21:45:30.677002+00:00 | codex | RESTORED_ALBUMIN_COUNTERPART_IDENTITIES_AND_STOCK_CONCENTRATIONS_WITH_EVIDENCE | Restore albumin and remove false LB constituents; correct mass/dose and stock/receiving-batch scopes. Retain exact source batch masses without assumed final volume. Unspecified percentage bases remain unknown. No growth claims or new chemical IDs are inferred. JCM252 supports 10% w/v albumin stock and a calculated 10 ml batch dose. |