Overview
- External ontology
- mediadive.medium:J266 (source link unavailable) — METHANOMICROBIUM MOBILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=266 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002623["methanomicrobium_mobile_medium"]
ing0_Rumen_fluid["Rumen fluid"]
CultureMech_002623 --> ing0_Rumen_fluid
ing1_KH2PO4["KH2PO4"]
CultureMech_002623 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_002623 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002623 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_NaCl["NaCl"]
CultureMech_002623 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002623 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002623 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002623 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_NaHCO3["NaHCO3"]
CultureMech_002623 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Yeast_extract["Yeast extract"]
CultureMech_002623 --> ing9_Yeast_extract
ing10_Trypticase_peptone["Trypticase peptone"]
CultureMech_002623 --> ing10_Trypticase_peptone
ing11_Resazurin["Resazurin"]
CultureMech_002623 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002623 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002623 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Valeric_acid["Valeric acid"]
CultureMech_002623 --> ing14_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing14_Valeric_acid -.-> CHEBI_17418
ing15_Isovaleric_acid["Isovaleric acid"]
CultureMech_002623 --> ing15_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing15_Isovaleric_acid -.-> CHEBI_28484
ing16_Tryptone["Tryptone"]
CultureMech_002623 --> ing16_Tryptone
ing17_Yeast_extract["Yeast extract"]
CultureMech_002623 --> ing17_Yeast_extract
ing18_Sodium_chloride["Sodium chloride"]
CultureMech_002623 --> ing18_Sodium_chloride
ing18_Sodium_chloride -.-> CHEBI_26710
ing19_Isobutyric_acid["Isobutyric acid"]
CultureMech_002623 --> ing19_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing19_Isobutyric_acid -.-> CHEBI_16135
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002623 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002623 --> ing21_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing21_MnSO4_x_n_H2O -.-> CHEBI_86360
ing22_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002623 --> ing22_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing22_CoSO4_x_7_H2O -.-> CHEBI_91244
ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002623 --> ing23_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002623 --> ing24_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
ing25_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002623 --> ing25_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing25_AlK_SO4_2 -.-> CHEBI_86463
ing26_H3BO3["H3BO3"]
CultureMech_002623 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002623 --> ing27_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing28_Biotin["Biotin"]
CultureMech_002623 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_002623 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_002623 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen fluid | 300 G_PER_L | ( clarified) | |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
1.6 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.6, 1.0] |
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.13 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.13, 3.0] |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.10800000000000001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.008, 0.1] |
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10200000000000001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.002, 0.1] |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 | Yeast extract | 1 G_PER_L | ||
| 11 | Trypticase peptone | 1 G_PER_L | ||
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 15 |
Valeric acid
C5H10O2 102.133 g/mol
|
25 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 16 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
25 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 17 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 18 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 19 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 20 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
25 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 22 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 23 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 24 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 25 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 26 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 28 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 35 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 36 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 37 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 38 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
- To prepare the clarified rumen fluid, preheat rumen content at 120C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.
- Adjust pH to 7.5 with concentrated NaOH solution.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J266_METHANOMICROBIUM_MOBILE_MEDIUM (CultureMech:002623)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.123934Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J266 |
| 2026-03-13T03:09:16.203375Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.597889Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002623 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.881317+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMICROBIUM MOBILE MEDIUM" to "methanomicrobium_mobile_medium" for consistent matching |
| 2026-06-05T07:40:16.934945+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.861096+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:20.929160+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 03954add7ffbd4f02605a6e5a8bfc86d6ef34caf260c8b4b34675fdce93c4355 |