Overview
- External ontology
- mediadive.medium:J266 (source link unavailable) — METHANOMICROBIUM MOBILE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=266 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The earlier LB commercial-product expansion is superseded: the exact recipe specifies a methylbutyric acid input instead. Remove only the reviewed LB supplier nodes, preserving genuine peptone, yeast extract and salt entries. Restore the acid at its source preparation stage, without inventing a chemical ID. Main-medium preparation specifies pH 6.5. API metadata min_pH/max_pH 7.5 conflicts and corresponds to the fatty-acid stock procedure; it is not selected for the medium.
Composition
flowchart LR
CultureMech_002623["methanomicrobium_mobile_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002623 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002623 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002623 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_NaCl["NaCl"]
CultureMech_002623 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002623 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002623 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002623 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_NaHCO3["NaHCO3"]
CultureMech_002623 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Yeast_extract["Yeast extract"]
CultureMech_002623 --> ing8_Yeast_extract
ing9_Trypticase_peptone["Trypticase peptone"]
CultureMech_002623 --> ing9_Trypticase_peptone
ing10_Resazurin["Resazurin"]
CultureMech_002623 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Distilled_water["Distilled water"]
CultureMech_002623 --> ing11_Distilled_water
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 0.3 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 0.3 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
CHEBI:62946
ammonium sulfate
|
Source batch input: 0.3 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.6, 1.0] Source batch input: 0.6 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. | |
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.13, 3.0] Source batch input: 0.13 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. | |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.008, 0.1] Source batch input: 8 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. | |
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.002, 0.1] Source batch input: 2 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. | |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
CHEBI:32139
sodium hydrogencarbonate
|
Source batch input: 2 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 9 | Yeast extract | Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-Difco. | ||
| 10 | Trypticase peptone | Source batch input: 1 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. Source qualifier: BD-BBL. | ||
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Source batch input: 1 mg. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. | |
| 12 | Distilled water | 0.66 L | Source water batch input: 660 ml = 0.66 L. This is not a measured final volume. |
Preparation
- Mix ingredients, except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
Solutions
Rumen fluid
Dose: 0.3 L
( clarified)
Source preparation input: 300 ml = 0.3 L per receiving batch, not grams per liter or a dose per final liter.
Trace minerals
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | Source stock batch input: 1.5 g. Retain provider-declared stock g_l=1.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| MgSO4 x 7 H2O | 3 G_PER_L | [Merged 2 duplicates: 0.13, 3.0] Source stock batch input: 3 g. Retain provider-declared stock g_l=3 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. |
| MnSO4 x n H2O | 0.5 G_PER_L | Source stock batch input: 0.5 g. Retain provider-declared stock g_l=0.5 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| NaCl | 1 G_PER_L | [Merged 2 duplicates: 0.6, 1.0] Source stock batch input: 1 g. Retain provider-declared stock g_l=1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. |
| FeSO4 x 7 H2O | 0.1 G_PER_L | [Merged 2 duplicates: 0.002, 0.1] Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. |
| CoSO4 x 7 H2O | 0.1 G_PER_L | Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CaCl2 x 2 H2O | 0.1 G_PER_L | [Merged 2 duplicates: 0.008, 0.1] Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. The earlier duplicate-summing note is superseded: main and trace-stock inputs are separate preparation scopes. This node retains only the source row at its stated stage. |
| ZnSO4 x 7 H2O | 0.1 G_PER_L | Source stock batch input: 0.1 g. Retain provider-declared stock g_l=0.1 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| CuSO4 x 5 H2O | 0.01 G_PER_L | Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| AlK(SO4)2 | 0.01 G_PER_L | Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| H3BO3 | 0.01 G_PER_L | Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Na2MoO4 x 2 H2O | 0.01 G_PER_L | Source stock batch input: 0.01 g. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. |
Source preparation input: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
KOH solution
Source-explicit stock pH-adjustment reagent with no fixed dose or numeric strength.
Trace vitamins
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.002 G_PER_L | Source stock batch input: 2 mg. Retain provider-declared stock g_l=0.002 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Folic acid | 0.002 G_PER_L | Source stock batch input: 2 mg. Retain provider-declared stock g_l=0.002 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Pyridoxine hydrochloride | 0.01 G_PER_L | Source stock batch input: 10 mg. Retain provider-declared stock g_l=0.01 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Thiamine HCl | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Riboflavin | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Nicotinic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Calcium pantothenate | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Vitamin B12 | 0.0001 G_PER_L | Source stock batch input: 0.1 mg. Retain provider-declared stock g_l=0.0001 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| p-Aminobenzoic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Lipoic acid | 0.005 G_PER_L | Source stock batch input: 5 mg. Retain provider-declared stock g_l=0.005 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 1 L | Source water batch input: 1000 ml = 1 L. This is not a measured final volume. |
Source preparation input: 10 ml = 0.01 L per receiving batch, not grams per liter or a dose per final liter.
Fatty acid mixture
Dose: 0.02 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Valeric acid | 25 G_PER_L | Source stock batch input: 0.5 g. Retain provider-declared stock g_l=25 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Isovaleric acid | 25 G_PER_L | Source stock batch input: 0.5 g. Retain provider-declared stock g_l=25 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| alpha-Methylbutyric acid | 25 G_PER_L | Source stock batch input: 0.5 g. Retain provider-declared stock g_l=25 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Isobutyric acid | 25 G_PER_L | Source stock batch input: 0.5 g. Retain provider-declared stock g_l=25 only, not a final-medium concentration or an independently verified final stock volume. Prior duplicate-merge notes describe the superseded flattened record. |
| Distilled water | 0.02 L | Source water batch input: 20 ml = 0.02 L. This is not a measured final volume. |
Source preparation input: 20 ml = 0.02 L per receiving batch, not grams per liter or a dose per final liter.
Concentrated NaOH solution
Source-explicit stock pH-adjustment reagent with no fixed dose or numeric strength.
Na2S x 9 H2O separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9 H2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
Separate preparation: composition notes retain the receiving-batch contribution, not a stock-batch recipe. Stock solvent volume, numeric strength and addition volume are unspecified. The source 5% label has unspecified percentage basis.
L-Cysteine HCl x H2O separately prepared solution
| Ingredient | Concentration | Notes |
|---|---|---|
| L-Cysteine HCl x H2O | Source batch input: 0.5 g. Final preparation volume is not independently established; the unsupported g/L assertion is withdrawn, not replaced by an assumed one-liter concentration. This is the contribution specified for the receiving medium batch, not the mass for preparing an unspecified volume of stock. |
Separate preparation: composition notes retain the receiving-batch contribution, not a stock-batch recipe. Stock solvent volume, numeric strength and addition volume are unspecified. The source 5% label has unspecified percentage basis.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.123934Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J266 |
| 2026-03-13T03:09:16.203375Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.597889Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002623 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.881317+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMICROBIUM MOBILE MEDIUM" to "methanomicrobium_mobile_medium" for consistent matching |
| 2026-06-05T07:40:16.934945+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.861096+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T20:41:03.546066+00:00 | codex | RESTORED_JCM_ACID_IDENTITIES_AND_PREPARATION_SCOPES_WITH_EVIDENCE | Superseded the erroneous LB expansion, restored source-specific fatty acids, stock definitions and doses, and retained exact preparation-stage amounts with supporting snippets. Preserved genuine same-labelled ingredients, unrelated annotations and append-only history. No final volume, percent basis, density or chemical grounding inferred. |