Overview
- External ontology
- mediadive.medium:J267 (source link unavailable) — AN1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=267
Composition
flowchart LR
CultureMech_002624["an1_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002624 --> ing0_Trypticase_peptone
ing1_K2HPO4["K2HPO4"]
CultureMech_002624 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaCl["NaCl"]
CultureMech_002624 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_002624 --> ing3_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing3_Sodium_thioglycolate -.-> CHEBI_86481
ing4_Sulfur["Sulfur"]
CultureMech_002624 --> ing4_Sulfur
CHEBI_26833["CHEBI:26833"]
ing4_Sulfur -.-> CHEBI_26833
ing5_Resazurin["Resazurin"]
CultureMech_002624 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 10 G_PER_L | ||
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
1 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 5 |
Sulfur
S 32.067 g/mol
|
8 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
Preparation
- Mix ingredients, except sodium thioglycolate and sulfur, and adjust pH to 7.3. Autoclave the medium under a N2 atmosphere. Separately autoclave sodium thioglycolate as 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the thioglycolate solution to the medium anaerobically.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J267_AN1_MEDIUM (CultureMech:002624)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.129921Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J267 |
| 2026-03-13T06:19:14.606417Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002624 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.890842+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AN1 MEDIUM" to "an1_medium" for consistent matching |
| 2026-06-05T07:40:16.946461+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.869342+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.891494+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b1b75d60b1accad4322b7663a27863e9d96312089f7a7ab4279f0464995ae143 |