an1_medium

Overview

External ontology

mediadive.medium:J267 (source link unavailable) — AN1 MEDIUM

Applications

• Microbial cultivation

pH

7.3

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=267

Composition

flowchart LR
    CultureMech_002624["an1_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002624 --> ing0_Trypticase_peptone
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002624 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_002624 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002624 --> ing3_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing3_Sodium_thioglycolate -.-> CHEBI_86481
    ing4_Sulfur["Sulfur"]
    CultureMech_002624 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833
    ing5_Resazurin["Resazurin"]
    CultureMech_002624 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806

Ingredients (6)

Preparation

1. Mix ingredients, except sodium thioglycolate and sulfur, and adjust pH to 7.3. Autoclave the medium under a N2 atmosphere. Separately autoclave sodium thioglycolate as 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the thioglycolate solution to the medium anaerobically.

Target organisms

No target organisms are recorded.

Provenance