Overview
- External ontology
- mediadive.medium:J282 (source link unavailable) — COLUMBIA BLOOD AGAR WITH 5% HORSE BLOOD
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'columbia_blood_agar_with_10_horse_blood', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J218', 'original_category': 'bacterial'}
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=282
Composition
flowchart LR
CultureMech_002640["columbia_blood_agar_with_5_horse_blood"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_002640 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_animal_tissue["Peptic digest of animal tissue"]
CultureMech_002640 --> ing1_Peptic_digest_of_animal_tissue
ing2_Beef_extract["Beef extract"]
CultureMech_002640 --> ing2_Beef_extract
ing3_Yeast_extract["Yeast extract"]
CultureMech_002640 --> ing3_Yeast_extract
ing4_Corn_starch["Corn starch"]
CultureMech_002640 --> ing4_Corn_starch
CHEBI_28017["CHEBI:28017"]
ing4_Corn_starch -.-> CHEBI_28017
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_002640 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_002640 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Defibrinated_horse_blood["Defibrinated horse blood"]
CultureMech_002640 --> ing7_Defibrinated_horse_blood
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Pancreatic digest of casein | 10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
From Oxoid CM331 (Columbia Agar Base); Peptone source providing amino acids and nitrogen |
| 2 | Peptic digest of animal tissue | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
From Oxoid CM331 (Columbia Agar Base); Peptone providing nutrients |
| 3 | Beef extract | 3.0 G_PER_L |
FOODON:03302088
beef extract
|
From Oxoid CM331 (Columbia Agar Base); Provides vitamins, minerals, and growth factors |
| 4 | Yeast extract | 5.0 G_PER_L |
FOODON:03315426
yeast extract
|
From Oxoid CM331 (Columbia Agar Base); Vitamin and amino acid source |
| 5 | Corn starch | 1.0 G_PER_L |
CHEBI:28017
starch
|
From Oxoid CM331 (Columbia Agar Base); Detoxifies metabolic byproducts |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
From Oxoid CM331 (Columbia Agar Base); Maintains osmotic balance |
| 7 | Agar | 13.5 G_PER_L |
CHEBI:2509
agar
|
From Oxoid CM331 (Columbia Agar Base); Solidifying agent |
| 8 | Defibrinated horse blood | 10 PERCENT_V_V |
UBERON:0000178
blood
|
Added aseptically after autoclaving and cooling to 45-50°C; 10% final concentration |
Preparation
- Prepare Columbia blood agar base (Oxoid CM331) according to the direction, sterilize and cool to about 45C. Aseptically add 5% (final) sterile defibrinated horse blood. Mix and quickly dispense into sterile test tubes or petri dishes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- columbia_blood_agar_with_10_horse_blood (CultureMech:002579)
- columbia_blood_agar_with_5_horse_blood (CultureMech:002640)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.173074Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J282 |
| 2026-02-03T11:26:54.397226 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:14.717561Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002640 |
| 2026-03-17T19:45:28.014208+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "COLUMBIA BLOOD AGAR WITH 5% HORSE BLOOD" to "columbia_blood_agar_with_5_horse_blood" for consistent matching |
| 2026-04-04T21:05:16.919246 | expand_columbia_blood_agar | EXPANDED_COMMERCIAL_PRODUCT | Commercial product expansion: Columbia Agar Base (CM331) with horse blood |
| 2026-05-16T08:57:26.735784+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-08-06T03:05:28.999011+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: columbia_blood_agar_with_10_horse_blood, columbia_blood_agar_with_5_horse_blood |
| 2026-08-06T03:05:28.999057+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 65a36f666eb9394e683a29e16d90ca2f547fcd597c2ed06f478d1aeb209d5502 |