columbia_blood_agar_with_5_horse_blood

CultureMech:002640 bacterial COMPLEX LIQUID
Original name: COLUMBIA BLOOD AGAR WITH 5% HORSE BLOOD

Overview

External ontology
mediadive.medium:J282 (source link unavailable) — COLUMBIA BLOOD AGAR WITH 5% HORSE BLOOD
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=282

Composition

flowchart LR
    CultureMech_002640["columbia_blood_agar_with_5_horse_blood"]
    ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_002640 --> ing0_Pancreatic_digest_of_casein
    ing1_Peptic_digest_of_animal_tissue["Peptic digest of animal tissue"]
    CultureMech_002640 --> ing1_Peptic_digest_of_animal_tissue
    ing2_Beef_extract["Beef extract"]
    CultureMech_002640 --> ing2_Beef_extract
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002640 --> ing3_Yeast_extract
    ing4_Corn_starch["Corn starch"]
    CultureMech_002640 --> ing4_Corn_starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Corn_starch -.-> CHEBI_28017
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_002640 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_002640 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Defibrinated_horse_blood["Defibrinated horse blood"]
    CultureMech_002640 --> ing7_Defibrinated_horse_blood

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Pancreatic digest of casein 10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 From Oxoid CM331 (Columbia Agar Base); Peptone source providing amino acids and nitrogen
2 Peptic digest of animal tissue 5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 From Oxoid CM331 (Columbia Agar Base); Peptone providing nutrients
3 Beef extract 3.0 G_PER_L FOODON:03302088
beef extract
 From Oxoid CM331 (Columbia Agar Base); Provides vitamins, minerals, and growth factors
4 Yeast extract 5.0 G_PER_L FOODON:03315426
yeast extract
 From Oxoid CM331 (Columbia Agar Base); Vitamin and amino acid source
5 Corn starch 1.0 G_PER_L CHEBI:28017
starch
 From Oxoid CM331 (Columbia Agar Base); Detoxifies metabolic byproducts
6 Sodium chloride
Cl.Na 58.443 g/mol
5.0 G_PER_L CHEBI:26710
sodium chloride
 From Oxoid CM331 (Columbia Agar Base); Maintains osmotic balance
7 Agar 13.5 G_PER_L CHEBI:2509
agar
 From Oxoid CM331 (Columbia Agar Base); Solidifying agent
8 Defibrinated horse blood 5 PERCENT_V_V UBERON:0000178
blood
 Added aseptically after autoclaving and cooling to 45-50°C; 5% final concentration

Preparation

  1. Prepare Columbia blood agar base (Oxoid CM331) according to the direction, sterilize and cool to about 45C. Aseptically add 5% (final) sterile defibrinated horse blood. Mix and quickly dispense into sterile test tubes or petri dishes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.173074Z mediadive-import Imported from MediaDive Source: JCM, ID: J282
2026-02-03T11:26:54.397226 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:14.717561Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002640
2026-03-17T19:45:28.014208+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "COLUMBIA BLOOD AGAR WITH 5% HORSE BLOOD" to "columbia_blood_agar_with_5_horse_blood" for consistent matching
2026-04-04T21:05:16.919246 expand_columbia_blood_agar EXPANDED_COMMERCIAL_PRODUCT Commercial product expansion: Columbia Agar Base (CM331) with horse blood
2026-05-16T08:57:26.735784+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(3 entries)
2026-09-06T00:00:00-07:00 repair_columbia_blood_score40.py LINKED_COLUMBIA_BLOOD_SCORE40_SOURCE_DUPLICATE Added or refreshed reciprocal source-duplicate link for bacterial/TOGO_M276_Columbia_Blood_Agar_With_5_Horse_Blood.yaml.