Overview
- External ontology
- mediadive.medium:J298 (source link unavailable) — HALOSIMPLEX MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=298
Composition
flowchart LR
CultureMech_002655["halosimplex_medium"]
ing0_Glycerol["Glycerol"]
CultureMech_002655 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002655 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_K2HPO4["K2HPO4"]
CultureMech_002655 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002655 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_KCl["KCl"]
CultureMech_002655 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_002655 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002655 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002655 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002655 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_002655 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002655 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002655 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002655 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002655 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
1250 G_PER_L |
CHEBI:17754
glycerol
|
|
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1250 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
250 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
500 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2000 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
100000 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10000 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.2 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15.
- For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco).
- Adjust pH to 3.0 - 4.0 with HCl.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halosimplex_medium (CultureMech:002655)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.215663Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J298 |
| 2026-03-13T06:19:14.804990Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002655 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.112494+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOSIMPLEX MEDIUM" to "halosimplex_medium" for consistent matching |
| 2026-06-05T07:41:08.222144+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.671382+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.979017+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bc49b9b53422a1f2945f25ebb45a083b4a3faa3e91b4ccfe802231623707d1e1 |