halosimplex_medium

CultureMech:002655 archaea DEFINED LIQUID
Original name: HALOSIMPLEX MEDIUM

Overview

External ontology
mediadive.medium:J298 (source link unavailable) — HALOSIMPLEX MEDIUM
Applications
  • Microbial cultivation
pH
3.5
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=298

Composition

flowchart LR
    CultureMech_002655["halosimplex_medium"]
    ing0_Glycerol["Glycerol"]
    CultureMech_002655 --> ing0_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing0_Glycerol -.-> CHEBI_17754
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002655 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002655 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002655 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_KCl["KCl"]
    CultureMech_002655 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaCl["NaCl"]
    CultureMech_002655 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002655 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002655 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002655 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002655 --> ing9_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002655 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Glycerol
C3H8O3 92.094 g/mol
1250 G_PER_L CHEBI:17754
glycerol
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1250 G_PER_L CHEBI:50144
sodium pyruvate
3 K2HPO4
HO4P.2K 174.174 g/mol
250 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
500 G_PER_L CHEBI:62946
ammonium sulfate
5 KCl
Cl.K 74.551 g/mol
2000 G_PER_L CHEBI:32588
potassium chloride
6 NaCl
Cl.Na 58.443 g/mol
100000 G_PER_L CHEBI:26710
sodium chloride
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10000 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.3 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.2 G_PER_L CHEBI:34887
nickel dichloride
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.3 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15.
  2. For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco).
  3. Adjust pH to 3.0 - 4.0 with HCl.

Solutions

Trace metal solution

Dose: 2 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 1 G_PER_L
H3BO3 3 G_PER_L
CoCl2 x 6 H2O 2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.215663Z mediadive-import Imported from MediaDive Source: JCM, ID: J298
2026-03-13T06:19:14.804990Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002655
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.112494+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOSIMPLEX MEDIUM" to "halosimplex_medium" for consistent matching
2026-06-05T07:41:08.222144+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.671382+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.220331+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace metal solution' @ 2 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).