Overview
- External ontology
- mediadive.medium:J299 (source link unavailable) — RC+ MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=299
Composition
flowchart LR
CultureMech_002656["rc_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002656 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002656 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002656 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_002656 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_002656 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Sodium_acetate["Sodium acetate"]
CultureMech_002656 --> ing5_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Sodium_acetate -.-> CHEBI_32954
ing6_MES["MES"]
CultureMech_002656 --> ing6_MES
CHEBI_39010["CHEBI:39010"]
ing6_MES -.-> CHEBI_39010
ing7_Yeast_extract["Yeast extract"]
CultureMech_002656 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_002656 --> ing8_Trypticase_peptone
ing9_K2HPO4["K2HPO4"]
CultureMech_002656 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_KH2PO4["KH2PO4"]
CultureMech_002656 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Resazurin["Resazurin"]
CultureMech_002656 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_L_Cystine["L-Cystine"]
CultureMech_002656 --> ing12_L_Cystine
CHEBI_16283["CHEBI:16283"]
ing12_L_Cystine -.-> CHEBI_16283
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002656 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_002656 --> ing14_Maltose_monohydrate
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.83 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 | MES | 2.13 G_PER_L |
CHEBI:39010
MES
|
|
| 8 | Yeast extract | 5 G_PER_L | ||
| 9 | Trypticase peptone | 5 G_PER_L | ||
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
L-Cystine
C6H12N2O4S2 240.306 g/mol
|
12 G_PER_L |
CHEBI:16283
L-cystine
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 | Maltose monohydrate | 7.2 G_PER_L |
Preparation
- Mix ingredients, except Na2S x 9H2O and L-cystine, and adjust pH to 6.0 with H2SO4. Fill the gas phase with N2, seal with butyl rubber stopper, and autoclave. Separately autoclave Na2S x 9H2O and L-cystine as 5% and 12% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cystine solutions, and readjust pH to 6.0, if necessary. Pressurize the inoculated bottles to 300 kPa N2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rc_medium (CultureMech:002656)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.218385Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J299 |
| 2026-03-13T06:19:14.812341Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002656 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.120596+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RC+ MEDIUM" to "rc_medium" for consistent matching |
| 2026-06-05T07:41:43.893996+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.864872+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.352330+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9776cd83ea6eff6bde6613f0aed0cdf14802c3f0b65cfb0169a061743b29926f |