rc_medium

CultureMech:002656 bacterial COMPLEX LIQUID
Original name: RC+ MEDIUM

Overview

External ontology
mediadive.medium:J299 (source link unavailable) — RC+ MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=299

Composition

flowchart LR
    CultureMech_002656["rc_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002656 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002656 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002656 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_002656 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaCl["NaCl"]
    CultureMech_002656 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Sodium_acetate["Sodium acetate"]
    CultureMech_002656 --> ing5_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Sodium_acetate -.-> CHEBI_32954
    ing6_MES["MES"]
    CultureMech_002656 --> ing6_MES
    CHEBI_39010["CHEBI:39010"]
    ing6_MES -.-> CHEBI_39010
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002656 --> ing7_Yeast_extract
    ing8_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002656 --> ing8_Trypticase_peptone
    ing9_K2HPO4["K2HPO4"]
    CultureMech_002656 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_KH2PO4["KH2PO4"]
    CultureMech_002656 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_Resazurin["Resazurin"]
    CultureMech_002656 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_L_Cystine["L-Cystine"]
    CultureMech_002656 --> ing12_L_Cystine
    CHEBI_16283["CHEBI:16283"]
    ing12_L_Cystine -.-> CHEBI_16283
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002656 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Maltose_monohydrate["Maltose monohydrate"]
    CultureMech_002656 --> ing14_Maltose_monohydrate

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
6 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.83 G_PER_L CHEBI:32954
sodium acetate
7 MES 2.13 G_PER_L CHEBI:39010
MES
8 Yeast extract 5 G_PER_L
9 Trypticase peptone 5 G_PER_L
10 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
11 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
13 L-Cystine
C6H12N2O4S2 240.306 g/mol
12 G_PER_L CHEBI:16283
L-cystine
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Maltose monohydrate 7.2 G_PER_L

Preparation

  1. Mix ingredients, except Na2S x 9H2O and L-cystine, and adjust pH to 6.0 with H2SO4. Fill the gas phase with N2, seal with butyl rubber stopper, and autoclave. Separately autoclave Na2S x 9H2O and L-cystine as 5% and 12% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cystine solutions, and readjust pH to 6.0, if necessary. Pressurize the inoculated bottles to 300 kPa N2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.218385Z mediadive-import Imported from MediaDive Source: JCM, ID: J299
2026-03-13T06:19:14.812341Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002656
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.120596+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RC+ MEDIUM" to "rc_medium" for consistent matching
2026-06-05T07:41:43.893996+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.864872+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.