Overview
- External ontology
- mediadive.medium:J314 (source link unavailable) — JCM MEDIUM No. 314
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=314
Composition
flowchart LR
CultureMech_002672["jcm_medium_no_314"]
ing0_Na2CO3["Na2CO3"]
CultureMech_002672 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_002672 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_002672 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Na2S2O3["Na2S2O3"]
CultureMech_002672 --> ing3_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing3_Na2S2O3 -.-> CHEBI_132112
ing4_KNO3["KNO3"]
CultureMech_002672 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002672 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_EDTA["EDTA"]
CultureMech_002672 --> ing6_EDTA
CHEBI_4735["CHEBI:4735"]
ing6_EDTA -.-> CHEBI_4735
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002672 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002672 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002672 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002672 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002672 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002672 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002672 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_H3BO3["H3BO3"]
CultureMech_002672 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
87.6339 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
19.4742 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
11.6845 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
20 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 5 |
KNO3
K.NO3 101.102 g/mol
|
5 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Preparation
- Filter-sterilize Na2S2O3 solution and KNO3 solution, and separately autoclave Trace elements solution and MgCl2 x 6H2O solution. Aseptically add Na2S2O3 solution, KNO3 solution, Trace elements solution and MgCl2 x 6H2O solution to the medium.
- Adjust pH to 3 with HCl.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_314 (CultureMech:002672)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.260330Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J314 |
| 2026-03-13T06:19:14.909798Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002672 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.227081+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 314" to "jcm_medium_no_314" for consistent matching |
| 2026-06-05T07:41:10.095150+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.023152+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.287667+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.263641+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a27f5a2138c1706c767593921b677b36871138ada6a52a9a81bc8f679df75e31 |