jcm_medium_no_314

CultureMech:002672 bacterial DEFINED LIQUID
Original name: JCM MEDIUM No. 314

Overview

External ontology
mediadive.medium:J314 (source link unavailable) — JCM MEDIUM No. 314
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=314

Composition

flowchart LR
    CultureMech_002672["jcm_medium_no_314"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_002672 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002672 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NaCl["NaCl"]
    CultureMech_002672 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Na2S2O3["Na2S2O3"]
    CultureMech_002672 --> ing3_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing3_Na2S2O3 -.-> CHEBI_132112
    ing4_KNO3["KNO3"]
    CultureMech_002672 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002672 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_EDTA["EDTA"]
    CultureMech_002672 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002672 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002672 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002672 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002672 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002672 --> ing11_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002672 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002672 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_H3BO3["H3BO3"]
    CultureMech_002672 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
87.6339 G_PER_L CHEBI:29377
sodium carbonate
2 NaHCO3
CHO3.Na 84.006 g/mol
19.4742 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NaCl
Cl.Na 58.443 g/mol
11.6845 G_PER_L CHEBI:26710
sodium chloride
4 Na2S2O3
2Na.O3S2 158.111 g/mol
20 G_PER_L CHEBI:132112
sodium thiosulfate
5 KNO3
K.NO3 101.102 g/mol
5 G_PER_L CHEBI:63043
potassium nitrate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid

Preparation

  1. Filter-sterilize Na2S2O3 solution and KNO3 solution, and separately autoclave Trace elements solution and MgCl2 x 6H2O solution. Aseptically add Na2S2O3 solution, KNO3 solution, Trace elements solution and MgCl2 x 6H2O solution to the medium.
  2. Adjust pH to 3 with HCl.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.260330Z mediadive-import Imported from MediaDive Source: JCM, ID: J314
2026-03-13T06:19:14.909798Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002672
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.227081+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 314" to "jcm_medium_no_314" for consistent matching
2026-06-05T07:41:10.095150+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.023152+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.287667+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.