Overview
- External ontology
- mediadive.medium:J327 (source link unavailable) — RENIBACTERIUM KDM-2 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=327
Composition
flowchart LR
CultureMech_002685["renibacterium_kdm_2_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_002685 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002685 --> ing1_Yeast_extract
ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002685 --> ing2_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing3_Agar["Agar"]
CultureMech_002685 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_002685 --> ing4_Fetal_bovine_serum
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 5 | Fetal bovine serum | 200 G_PER_L |
Preparation
- Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. After autoclaving, cool to 45C and add serum.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- renibacterium_kdm_2_medium (CultureMech:002685)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.307591Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J327 |
| 2026-03-13T06:19:15.026185Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002685 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.362818+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RENIBACTERIUM KDM-2 MEDIUM" to "renibacterium_kdm_2_medium" for consistent matching |
| 2026-06-05T07:41:44.165124+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:54.578815+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e65e829610d63e020714de84b101a8eb7a4a4afcfbb42af0d9423c524f3a0ba6 |