renibacterium_kdm_2_medium

CultureMech:002685 bacterial COMPLEX SOLID_AGAR
Original name: RENIBACTERIUM KDM-2 MEDIUM

Overview

External ontology
mediadive.medium:J327 (source link unavailable) — RENIBACTERIUM KDM-2 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=327

Composition

flowchart LR
    CultureMech_002685["renibacterium_kdm_2_medium"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_002685 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002685 --> ing1_Yeast_extract
    ing2_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002685 --> ing2_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing2_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing3_Agar["Agar"]
    CultureMech_002685 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_002685 --> ing4_Fetal_bovine_serum

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto peptone 10 G_PER_L MICRO:0000178 (source link unavailable)
Bacto peptone
2 Yeast extract 0.5 G_PER_L FOODON:03315426
Yeast extract
3 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
1 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
4 Agar 15 G_PER_L CHEBI:2509
agar
5 Fetal bovine serum 200 G_PER_L

Preparation

  1. Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. After autoclaving, cool to 45C and add serum.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.307591Z mediadive-import Imported from MediaDive Source: JCM, ID: J327
2026-03-13T06:19:15.026185Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002685
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.362818+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RENIBACTERIUM KDM-2 MEDIUM" to "renibacterium_kdm_2_medium" for consistent matching
2026-06-05T07:41:44.165124+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_bacterial_score10_exact_terms_batch7.py GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH7 Applied local mappings for Bacto peptone, Yeast extract.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.