r2a_agar

CultureMech:002706 bacterial COMPLEX SOLID_AGAR
Original name: R2A AGAR

Overview

External ontology
mediadive.medium:J346 (source link unavailable) — R2A AGAR
Applications
  • Microbial cultivation
Synonyms
  • {'name': '5_x_r2a_agar', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1091', 'original_category': 'bacterial'}
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=346

Composition

flowchart LR
    CultureMech_002706["r2a_agar"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002706 --> ing0_Yeast_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_002706 --> ing1_Proteose_peptone_no__3
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_002706 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_002706 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_002706 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002706 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002706 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002706 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_002706 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2.5 G_PER_L
2 Proteose peptone no. 3 2.5 G_PER_L
3 Casamino acids 2.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17234
glucose
5 Starch 2.5 G_PER_L CHEBI:28017
starch
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1.5 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
1.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Adjust pH to 7.2.
  2. Comment: The premixed powder is available from Becton Dickinson & Co. as Difco R2A Agar.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:16.355571Z mediadive-import Imported from MediaDive Source: JCM, ID: J346
2026-01-28T19:48:11.905708Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:15.143089Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002706
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.498001+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R2A AGAR" to "r2a_agar" for consistent matching
2026-05-24T04:38:33.216229+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=1 env(s) from CommunityMech
2026-06-05T07:41:43.569932+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.989677+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:10.763018+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:34.176144+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: 5_x_r2a_agar, r2a_agar
2026-08-06T03:04:34.176186+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 81c555c62256335e9d3ca6e996cdabef06dbef8a7545ae750f337d50ca761da5