5_x_r2a_agar

CultureMech:002271 bacterial COMPLEX SOLID_AGAR
Original name: 5 x R2A AGAR

Overview

External ontology
mediadive.medium:J1091 (source link unavailable) — 5 x R2A AGAR
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1091 Composition verified against https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1091 on 2026-08-25 (SHA-256 3335c9e28273375ce3f454927ff1ed7d233af7b50869fb9f758d0e5451a944f5).

Composition

flowchart LR
    CultureMech_002271["5_x_r2a_agar"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002271 --> ing0_Yeast_extract
    ing1_Proteose_peptone_No__3["Proteose peptone No. 3"]
    CultureMech_002271 --> ing1_Proteose_peptone_No__3
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_002271 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_002271 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Soluble_starch["Soluble starch"]
    CultureMech_002271 --> ing4_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Soluble_starch -.-> CHEBI_28017
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002271 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002271 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002271 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_002271 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Distilled_water["Distilled water"]
    CultureMech_002271 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Yeast extract 2.5 G_PER_L FOODON:03315426
yeast extract
2 Proteose peptone No. 3 2.5 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
3 Casamino acids 2.5 G_PER_L FOODON:03315719
mammalian milk protein (hydrolyzed)
4 Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17234
glucose
5 Soluble starch 2.5 G_PER_L CHEBI:28017
starch
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
1.5 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
1.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Agar 15.0 G_PER_L CHEBI:2509
agar
10 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water

Preparation

  1. Combine the printed components with 1.0 L distilled water.
  2. Adjust the R2A base to pH 7.2.
  3. Autoclave at 121 C for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.597284Z mediadive-import Imported from MediaDive Source: JCM, ID: J1091
2026-01-28T19:48:28.936053Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:11.830305Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002271
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:24.703884+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "5 x R2A AGAR" to "5_x_r2a_agar" for consistent matching
2026-06-05T07:40:15.881116+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.181674+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.570367+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-25T00:00:00-07:00 repair_jcm_r2a_family.py RESTORED_JCM_R2A_FAMILY Restored the reviewed JCM R2A formulation, printed water, default sterilization, and reciprocal variant semantics. Identity objects were verified against the MIM SSSOM; unspecified carbonate volume remains explicitly variable.