Overview
- External ontology
- mediadive.medium:J355 (source link unavailable) — METHANOSPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=355
Composition
flowchart LR
CultureMech_002715["methanosphaera_medium"]
ing0_Rumen_fluid["Rumen fluid"]
CultureMech_002715 --> ing0_Rumen_fluid
ing1_Yeast_extract["Yeast extract"]
CultureMech_002715 --> ing1_Yeast_extract
ing2_Trypticase_peptone["Trypticase peptone"]
CultureMech_002715 --> ing2_Trypticase_peptone
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_002715 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Sodium_formate["Sodium formate"]
CultureMech_002715 --> ing4_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing4_Sodium_formate -.-> CHEBI_62965
ing5_Na2SeO4["Na2SeO4"]
CultureMech_002715 --> ing5_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing5_Na2SeO4 -.-> CHEBI_77775
ing6_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002715 --> ing6_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing6_NiCl2_x_6_H2O -.-> CHEBI_34887
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002715 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_KH2PO4["KH2PO4"]
CultureMech_002715 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_K2HPO4["K2HPO4"]
CultureMech_002715 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_002715 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_NH4Cl["NH4Cl"]
CultureMech_002715 --> ing11_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing11_NH4Cl -.-> CHEBI_31206
ing12_NaCl["NaCl"]
CultureMech_002715 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002715 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002715 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_Methanol["Methanol"]
CultureMech_002715 --> ing15_Methanol
CHEBI_17790["CHEBI:17790"]
ing15_Methanol -.-> CHEBI_17790
ing16_Resazurin["Resazurin"]
CultureMech_002715 --> ing16_Resazurin
CHEBI_8806["CHEBI:8806"]
ing16_Resazurin -.-> CHEBI_8806
ing17_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002715 --> ing17_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing17_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002715 --> ing18_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing18_Na2S_x_9_H2O -.-> CHEBI_76209
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002715 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002715 --> ing20_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing20_MnSO4_x_n_H2O -.-> CHEBI_86360
ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002715 --> ing21_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002715 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002715 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002715 --> ing24_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing24_AlK_SO4_2 -.-> CHEBI_86463
ing25_H3BO3["H3BO3"]
CultureMech_002715 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002715 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing27_Biotin["Biotin"]
CultureMech_002715 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_002715 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002715 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_002715 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen fluid | 100 G_PER_L | ( clarified) | |
| 2 | Yeast extract | 1.9685 G_PER_L | ||
| 3 | Trypticase peptone | 1.9685 G_PER_L | ||
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.492126 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.492126 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 6 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.000187008 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 7 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.000688976 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10295276 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00295276, 0.1] |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.75591 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.590551 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.295276 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 12 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.984252 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
1.590551 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.590551, 1.0] |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.147638 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.147638, 3.0] |
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1748032 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0748032, 0.1] |
| 16 |
Methanol
CH4O 32.042 g/mol
|
5 G_PER_L |
CHEBI:17790
methanol
|
|
| 17 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000984252 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 18 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.86122 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 19 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.369094 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 22 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 25 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 34 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 36 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 37 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except methanol, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of N2 and add methanol to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in a 100-ml serum bottle) under a stream of N2 and seal. After autoclaving, stand the medium overnight. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, replace the gas phase with H2-CO2 (80:20, v/v), and add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v). To prepare the clarified rumen fluid, preheat rumen content at 120C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J355_METHANOSPHAERA_MEDIUM (CultureMech:002715)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.377012Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J355 |
| 2026-03-13T03:09:32.401195Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:15.209000Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002715 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.568928+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSPHAERA MEDIUM" to "methanosphaera_medium" for consistent matching |
| 2026-06-05T07:40:17.103501+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.079911+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:42.914808+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:21.008419+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1bdcbb97bab6f92d29eb3c693e25d814ac847c865b9226093db6388e756b3702 |