Overview
- External ontology
- mediadive.medium:J355 (source link unavailable) — METHANOSPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=355
Composition
flowchart LR
CultureMech_002715["methanosphaera_medium"]
ing0_Rumen_fluid["Rumen fluid"]
CultureMech_002715 --> ing0_Rumen_fluid
ing1_Yeast_extract["Yeast extract"]
CultureMech_002715 --> ing1_Yeast_extract
ing2_Trypticase_peptone["Trypticase peptone"]
CultureMech_002715 --> ing2_Trypticase_peptone
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_002715 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Sodium_formate["Sodium formate"]
CultureMech_002715 --> ing4_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing4_Sodium_formate -.-> CHEBI_62965
ing5_Na2SeO4["Na2SeO4"]
CultureMech_002715 --> ing5_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing5_Na2SeO4 -.-> CHEBI_77775
ing6_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002715 --> ing6_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing6_NiCl2_x_6_H2O -.-> CHEBI_34887
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002715 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_KH2PO4["KH2PO4"]
CultureMech_002715 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_K2HPO4["K2HPO4"]
CultureMech_002715 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_002715 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_NH4Cl["NH4Cl"]
CultureMech_002715 --> ing11_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing11_NH4Cl -.-> CHEBI_31206
ing12_NaCl["NaCl"]
CultureMech_002715 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002715 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002715 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_Methanol["Methanol"]
CultureMech_002715 --> ing15_Methanol
CHEBI_17790["CHEBI:17790"]
ing15_Methanol -.-> CHEBI_17790
ing16_Resazurin["Resazurin"]
CultureMech_002715 --> ing16_Resazurin
CHEBI_8806["CHEBI:8806"]
ing16_Resazurin -.-> CHEBI_8806
ing17_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002715 --> ing17_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing17_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing18_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002715 --> ing18_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing18_Na2S_x_9_H2O -.-> CHEBI_76209
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002715 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002715 --> ing20_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing20_MnSO4_x_n_H2O -.-> CHEBI_86360
ing21_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002715 --> ing21_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing21_CoSO4_x_7_H2O -.-> CHEBI_91244
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002715 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002715 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002715 --> ing24_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing24_AlK_SO4_2 -.-> CHEBI_86463
ing25_H3BO3["H3BO3"]
CultureMech_002715 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002715 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing27_Biotin["Biotin"]
CultureMech_002715 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_Folic_acid["Folic acid"]
CultureMech_002715 --> ing28_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing28_Folic_acid -.-> CHEBI_27470
ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002715 --> ing29_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
CultureMech_002715 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen fluid | 100 G_PER_L | ( clarified) | |
| 2 | Yeast extract | 1.9685 G_PER_L | ||
| 3 | Trypticase peptone | 1.9685 G_PER_L | ||
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.492126 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.492126 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 6 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.000187008 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 7 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.000688976 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.10295276 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.00295276, 0.1] |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.75591 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.590551 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.295276 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 12 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.984252 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
1.590551 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.590551, 1.0] |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.147638 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.147638, 3.0] |
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1748032 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0748032, 0.1] |
| 16 |
Methanol
CH4O 32.042 g/mol
|
5 G_PER_L |
CHEBI:17790
methanol
|
|
| 17 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000984252 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 18 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.86122 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 19 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.369094 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 21 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 22 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 25 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 29 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 30 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 34 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 35 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 36 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 37 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients, except methanol, L-cysteine x HCl x H2O and Na2S x 9H2O, and adjust pH to 7.0. Bring to a boil for 5-10 sec, then, cool down under a stream of N2 and add methanol to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in a 100-ml serum bottle) under a stream of N2 and seal. After autoclaving, stand the medium overnight. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, replace the gas phase with H2-CO2 (80:20, v/v), and add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions aseptically and anaerobically. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v). To prepare the clarified rumen fluid, preheat rumen content at 120C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.377012Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J355 |
| 2026-03-13T03:09:32.401195Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:15.209000Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002715 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.568928+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSPHAERA MEDIUM" to "methanosphaera_medium" for consistent matching |
| 2026-06-05T07:40:17.103501+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.079911+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:42.914808+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |