Overview
- External ontology
- mediadive.medium:J413 (source link unavailable) — MMJS MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'mmjh_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J369', 'original_category': 'bacterial'}
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=413
Composition
flowchart LR
CultureMech_002769["mmjs_medium"]
ing0_NaCl["NaCl"]
CultureMech_002769 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_002769 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002769 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002769 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaNO3["NaNO3"]
CultureMech_002769 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002769 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002769 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_002769 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002769 --> ing8_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002769 --> ing9_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing10_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_002769 --> ing10_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing10_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing11_H2WO4["H2WO4"]
CultureMech_002769 --> ing11_H2WO4
CHEBI_36272["CHEBI:36272"]
ing11_H2WO4 -.-> CHEBI_36272
ing12_NaHCO3["NaHCO3"]
CultureMech_002769 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Biotin["Biotin"]
CultureMech_002769 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_002769 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002769 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_002769 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_Riboflavin["Riboflavin"]
CultureMech_002769 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_002769 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002769 --> ing19_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_pantothenate -.-> CHEBI_31345
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002769 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002769 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Lipoic_acid["Lipoic acid"]
CultureMech_002769 --> ing22_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing22_Lipoic_acid -.-> CHEBI_16494
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
29.6736 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138477 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.98912 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.24728 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.98912 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.36301 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13452 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.326409 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0049456 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 10 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049456 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 11 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
0.049456 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 12 |
H2WO4
H2O4W 249.852 g/mol
|
0.0049456 G_PER_L |
CHEBI:36272
tungstic acid
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.98912 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 20 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 21 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Use Medium No. 369 supplemented with 20.0 g/L (final) NaCl, 2.0 g/L (final) NaHCO3 and 2.0 g/L Na2S2O3 x 5H2O. Replace the gas phase with N2-CO2-O2 (79:20:1, v/v), and pressurized inoculated bottles to 200 kPa with the same gas mixture.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- mmjh_medium (CultureMech:002728)
- mmjs_medium (CultureMech:002769)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.545886Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J413 |
| 2026-02-03T11:26:54.850720 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:15.588464Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002769 |
| 2026-03-17T19:45:29.005870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJS MEDIUM" to "mmjs_medium" for consistent matching |
| 2026-04-04T18:49:19.554871 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from mmjh_medium (CultureMech:002728) |
| 2026-05-16T08:58:08.930882+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:41:35.638671+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.337928+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.314140+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: mmjh_medium, mmjs_medium |
| 2026-08-06T03:05:47.314191+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 4140db8f9c5213e5817fdcd6538abb9aace261f48bc8e96fce4089c2c80213d3 |