mmjh_medium

CultureMech:002728 bacterial DEFINED LIQUID
Original name: MMJH MEDIUM

Overview

External ontology
mediadive.medium:J369 (source link unavailable) — MMJH MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=369

Composition

flowchart LR
    CultureMech_002728["mmjh_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002728 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002728 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002728 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002728 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaNO3["NaNO3"]
    CultureMech_002728 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002728 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002728 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_002728 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002728 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002728 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_002728 --> ing10_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing10_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing11_H2WO4["H2WO4"]
    CultureMech_002728 --> ing11_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing11_H2WO4 -.-> CHEBI_36272
    ing12_NaHCO3["NaHCO3"]
    CultureMech_002728 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_Biotin["Biotin"]
    CultureMech_002728 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_002728 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002728 --> ing15_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002728 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_Riboflavin["Riboflavin"]
    CultureMech_002728 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002728 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002728 --> ing19_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_Calcium_pantothenate -.-> CHEBI_31345
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_002728 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002728 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Lipoic_acid["Lipoic acid"]
    CultureMech_002728 --> ing22_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing22_Lipoic_acid -.-> CHEBI_16494

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
29.6736 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.138477 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.98912 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.24728 G_PER_L CHEBI:31206
ammonium chloride
5 NaNO3
NO3.Na 84.994 g/mol
0.98912 G_PER_L CHEBI:63005
sodium nitrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.36301 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13452 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 KCl
Cl.K 74.551 g/mol
0.326409 G_PER_L CHEBI:32588
potassium chloride
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0049456 G_PER_L CHEBI:34887
nickel dichloride
10 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00049456 G_PER_L CHEBI:131361
disodium selenite pentahydrate
11 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
0.049456 G_PER_L CHEBI:53438
iron(3+) sulfate
12 H2WO4
H2O4W 249.852 g/mol
0.0049456 G_PER_L CHEBI:36272
tungstic acid
13 NaHCO3
CHO3.Na 84.006 g/mol
0.98912 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
15 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
16 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
17 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
18 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
20 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
21 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
22 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
23 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients except Fe2(SO4)3 x <I>x</I>H2O, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Separately autoclave 0.5% Fe2(SO4)3 x <I>x</I>H2O solution (in 0.1 N H2SO4), filter-sterilize Trace vitamins and 5% NaHCO3 solution and add the solutions to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2-O2 (80:20:1, v/v) gas mixture and seal with butyl rubber stoppers. Readjust pH to 6.8, if necessary. Pressurize the inoculated bottle to 200 kPa with the same gas mixture.
  2. Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.422606Z mediadive-import Imported from MediaDive Source: JCM, ID: J369
2026-03-13T06:19:15.317027Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002728
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.698995+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJH MEDIUM" to "mmjh_medium" for consistent matching
2026-06-05T07:41:35.576903+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.315010+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.