Overview
- External ontology
- mediadive.medium:J414 (source link unavailable) — MODIFIED SOB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=414
Composition
flowchart LR
CultureMech_002770["modified_sob_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002770 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002770 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002770 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002770 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002770 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002770 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_NaHCO3["NaHCO3"]
CultureMech_002770 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2_EDTA["Na2-EDTA"]
CultureMech_002770 --> ing7_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing7_Na2_EDTA -.-> CHEBI_64734
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002770 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002770 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_002770 --> ing10__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002770 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002770 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_NaOH["NaOH"]
CultureMech_002770 --> ing13_NaOH
CHEBI_32145["CHEBI:32145"]
ing13_NaOH -.-> CHEBI_32145
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1000 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1000 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1000 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
107.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 100.0, 7.3] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
202.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
[Merged 2 duplicates: 200.0, 2.5] |
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1550 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2000 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
2.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.5 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.5 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 14 |
NaOH
HO.Na 39.997 g/mol
|
11 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Preparation
- Dissolve KH2PO4, K2HPO4, NH4Cl and Na2S2O3 x 5H2O in 500 ml distilled water. Separately dissolve CaCl2 x 2H2O, MgCl2 x 6H2O and Trace metal solution in 460 ml distilled water. Autoclave both solutions, mix them after cooling, and add 40 ml filter-sterilized 5% NaHCO3 solution to the medium. Adjust pH to 7.0 with HCl, if necessary.
- Adjust pH to 6.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_sob_medium (CultureMech:002770)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.547235Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J414 |
| 2026-03-13T03:08:52.975477Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:15.594426Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002770 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.012209+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED SOB MEDIUM" to "modified_sob_medium" for consistent matching |
| 2026-06-05T07:41:37.755469+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.943725+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.778267+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 92ef7835ab50c895afc64a798d42cf80ce353b45b2961bcb9e8b8e0e843ceaf7 |