modified_sob_medium

CultureMech:002770 bacterial DEFINED LIQUID
Original name: MODIFIED SOB MEDIUM

Overview

External ontology
mediadive.medium:J414 (source link unavailable) — MODIFIED SOB MEDIUM
Applications
  • Microbial cultivation
pH
6.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=414

Composition

flowchart LR
    CultureMech_002770["modified_sob_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002770 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002770 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002770 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002770 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002770 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002770 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002770 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_002770 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002770 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002770 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_002770 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002770 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002770 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_NaOH["NaOH"]
    CultureMech_002770 --> ing13_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing13_NaOH -.-> CHEBI_32145

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1000 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
1000 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1000 G_PER_L CHEBI:31206
ammonium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
107.3 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 100.0, 7.3]
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
202.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 [Merged 2 duplicates: 200.0, 2.5]
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
1550 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
2000 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
50 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
2.2 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.5 G_PER_L CHEBI:91249
ammonium molybdate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 NaOH
HO.Na 39.997 g/mol
11 G_PER_L CHEBI:32145
sodium hydroxide

Preparation

  1. Dissolve KH2PO4, K2HPO4, NH4Cl and Na2S2O3 x 5H2O in 500 ml distilled water. Separately dissolve CaCl2 x 2H2O, MgCl2 x 6H2O and Trace metal solution in 460 ml distilled water. Autoclave both solutions, mix them after cooling, and add 40 ml filter-sterilized 5% NaHCO3 solution to the medium. Adjust pH to 7.0 with HCl, if necessary.
  2. Adjust pH to 6.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.547235Z mediadive-import Imported from MediaDive Source: JCM, ID: J414
2026-03-13T03:08:52.975477Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:15.594426Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002770
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.012209+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED SOB MEDIUM" to "modified_sob_medium" for consistent matching
2026-06-05T07:41:37.755469+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.943725+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.