pyg_medium_b

CultureMech:002776 bacterial COMPLEX LIQUID
Original name: PYG MEDIUM (B)

Overview

External ontology
mediadive.medium:J423 (source link unavailable) — PYG MEDIUM (B)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'pyg_medium_c', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J456', 'original_category': 'bacterial'}
  • {'name': 'pyg_medium_d', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J564', 'original_category': 'bacterial'}
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=423

Composition

flowchart LR
    CultureMech_002776["pyg_medium_b"]
    ing0_Polypeptone["Polypeptone"]
    CultureMech_002776 --> ing0_Polypeptone
    ing1_Tryptone["Tryptone"]
    CultureMech_002776 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002776 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_002776 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_CaCl2["CaCl2"]
    CultureMech_002776 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002776 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002776 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_KH2PO4["KH2PO4"]
    CultureMech_002776 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002776 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_NaCl["NaCl"]
    CultureMech_002776 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Polypeptone 5 G_PER_L
2 Tryptone 5 G_PER_L
3 Yeast extract 10 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
5 CaCl2
Ca.2Cl 110.984 g/mol
0.2 G_PER_L CHEBI:3312
calcium dichloride
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Adjust pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

Merged from 5 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:16.577675Z mediadive-import Imported from MediaDive Source: JCM, ID: J423
2026-03-13T06:19:15.654347Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002776
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.079975+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYG MEDIUM (B)" to "pyg_medium_b" for consistent matching
2026-06-05T07:40:17.238386+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.958652+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:36.264761+00:00 recipe-merger Merged 5 duplicate recipes into canonical record Sources: JCM_J423_PYG_MEDIUM_B, KOMODO_1139_PYG_medium_B, pyg_medium_b, pyg_medium_c, pyg_medium_d
2026-08-06T03:04:36.264831+00:00 merge_recipes.py MERGED_RECIPES merged 5 source recipe(s) on fingerprint 664c12a8c00a22ffbaef33dd2f268520eeccc3466cd7c2bdead67b103bee60e4