pyg_medium_d

Overview

External ontology

mediadive.medium:J564 (source link unavailable) — PYG MEDIUM (D)

Applications

• Microbial cultivation

pH

6.9

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=564

Composition

flowchart LR
    CultureMech_002913["pyg_medium_d"]
    ing0_Polypeptone["Polypeptone"]
    CultureMech_002913 --> ing0_Polypeptone
    ing1_Tryptone["Tryptone"]
    CultureMech_002913 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002913 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_002913 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_CaCl2["CaCl2"]
    CultureMech_002913 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002913 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002913 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_KH2PO4["KH2PO4"]
    CultureMech_002913 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002913 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_NaCl["NaCl"]
    CultureMech_002913 --> ing9_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing9_NaCl -.-> CHEBI_26710

Ingredients (10)

Preparation

1. Prepare Medium No. 423 and adjust pH to 6.9. Add 0.5 g/L L-cysteine x HCl x H2O after the medium is boiled, then, dispense into culture vessels. Prepare the medium under a N2 atmosphere.

Target organisms

No target organisms are recorded.

Provenance