rt2_medium

CultureMech:002789 bacterial COMPLEX LIQUID
Original name: RT2 MEDIUM

Overview

External ontology
mediadive.medium:J438 (source link unavailable) — RT2 MEDIUM
Applications
  • Microbial cultivation
pH
9.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=438

Composition

flowchart LR
    CultureMech_002789["rt2_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002789 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002789 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002789 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002789 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_002789 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_002789 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002789 --> ing6_Yeast_extract
    ing7_CAPSO["CAPSO"]
    CultureMech_002789 --> ing7_CAPSO
    CHEBI_183882["CHEBI:183882"]
    ing7_CAPSO -.-> CHEBI_183882
    ing8_Na2SO4["Na2SO4"]
    CultureMech_002789 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_002789 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_Resazurin["Resazurin"]
    CultureMech_002789 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Sodium_dithionite["Sodium dithionite"]
    CultureMech_002789 --> ing11_Sodium_dithionite
    CHEBI_66870["CHEBI:66870"]
    ing11_Sodium_dithionite -.-> CHEBI_66870
    ing12_NaOH["NaOH"]
    CultureMech_002789 --> ing12_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing12_NaOH -.-> CHEBI_32145
    ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002789 --> ing13_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002789 --> ing14_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
500 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
50 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
25 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
125 G_PER_L CHEBI:31206
ammonium chloride
5 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 KCl
Cl.K 74.551 g/mol
250 G_PER_L CHEBI:32588
potassium chloride
7 Yeast extract 250 G_PER_L
8 CAPSO
C9H19NO4S 237.321 g/mol
5930 G_PER_L CHEBI:183882
3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
9 Na2SO4
2Na.O4S 142.043 g/mol
2000 G_PER_L CHEBI:32149
sodium sulfate
10 Sodium lactate
C3H5O3.Na 112.06 g/mol
1120 G_PER_L CHEBI:75228
sodium lactate
11 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
12 Sodium dithionite
2Na.O4S2 174.11 g/mol
50 G_PER_L CHEBI:66870
sodium dithionite
13 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
14 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
15 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.

Solutions

Trace element solution

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.616464Z mediadive-import Imported from MediaDive Source: JCM, ID: J438
2026-01-28T19:48:42.593505Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:11.780583+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:15.725130Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002789
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.158300+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RT2 MEDIUM" to "rt2_medium" for consistent matching
2026-05-16T09:04:30.914811+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:44.730135+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.103922+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:54.909871+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint df06dcd87f47c4ed517bc58ea731510cc9174b8ab82573b9b12b913826543529