rt2_medium (CultureMech:002789)

Overview

External ontology

mediadive.medium:J438 (source link unavailable) — RT2 MEDIUM

Applications

Microbial cultivation

pH

9.0

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=438

Composition

flowchart LR
    CultureMech_002789["rt2_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002789 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002789 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002789 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002789 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_002789 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_KCl["KCl"]
    CultureMech_002789 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002789 --> ing6_Yeast_extract
    ing7_CAPSO["CAPSO"]
    CultureMech_002789 --> ing7_CAPSO
    CHEBI_183882["CHEBI:183882"]
    ing7_CAPSO -.-> CHEBI_183882
    ing8_Na2SO4["Na2SO4"]
    CultureMech_002789 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_002789 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_Resazurin["Resazurin"]
    CultureMech_002789 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Sodium_dithionite["Sodium dithionite"]
    CultureMech_002789 --> ing11_Sodium_dithionite
    CHEBI_66870["CHEBI:66870"]
    ing11_Sodium_dithionite -.-> CHEBI_66870
    ing12_NaOH["NaOH"]
    CultureMech_002789 --> ing12_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing12_NaOH -.-> CHEBI_32145
    ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002789 --> ing13_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002789 --> ing14_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (15)

#	Ingredient	Concentration	Ontology
1	NaCl Cl.Na 58.443 g/mol	500 G_PER_L	CHEBI:26710 sodium chloride
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	50 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
3	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	25 G_PER_L	CHEBI:86158 calcium chloride dihydrate
4	NH4Cl Cl.H4N 53.492 g/mol	125 G_PER_L	CHEBI:31206 ammonium chloride
5	KH2PO4 H2O4P.K 136.084 g/mol	50 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
6	KCl Cl.K 74.551 g/mol	250 G_PER_L	CHEBI:32588 potassium chloride
7	Yeast extract	250 G_PER_L
8	CAPSO C9H19NO4S 237.321 g/mol	5930 G_PER_L	CHEBI:183882 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid
9	Na2SO4 2Na.O4S 142.043 g/mol	2000 G_PER_L	CHEBI:32149 sodium sulfate
10	Sodium lactate C3H5O3.Na 112.06 g/mol	1120 G_PER_L	CHEBI:75228 sodium lactate
11	Resazurin C12H7NO4 229.191 g/mol	0.5 G_PER_L	CHEBI:8806 Resazurin
12	Sodium dithionite 2Na.O4S2 174.11 g/mol	50 G_PER_L	CHEBI:66870 sodium dithionite
13	NaOH HO.Na 39.997 g/mol	0.4 G_PER_L	CHEBI:32145 sodium hydroxide
14	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.006 G_PER_L	CHEBI:131361 disodium selenite pentahydrate
15	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.008 G_PER_L	CHEBI:63939 sodium tungstate dihydrate

Preparation

Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.

Solutions

Trace element solution

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.616464Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J438
2026-01-28T19:48:42.593505Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:11.780583+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:19:15.725130Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002789
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:56.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.158300+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "RT2 MEDIUM" to "rt2_medium" for consistent matching
2026-05-16T09:04:30.914811+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:41:44.730135+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.103922+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.