ka22_medium

CultureMech:002798 bacterial COMPLEX LIQUID
Original name: KA22 MEDIUM

Overview

External ontology
mediadive.medium:J448 (source link unavailable) — KA22 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=448

Composition

flowchart LR
    CultureMech_002798["ka22_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_002798 --> ing0_Sea_Salt
    ing1_Mg_NO3_2_x_6_H2O["Mg(NO3)2 x 6 H2O"]
    CultureMech_002798 --> ing1_Mg_NO3_2_x_6_H2O
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002798 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaHCO3["NaHCO3"]
    CultureMech_002798 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_MES["MES"]
    CultureMech_002798 --> ing4_MES
    CHEBI_39010["CHEBI:39010"]
    ing4_MES -.-> CHEBI_39010
    ing5_Sulfur["Sulfur"]
    CultureMech_002798 --> ing5_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing5_Sulfur -.-> CHEBI_26833
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002798 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_Resazurin["Resazurin"]
    CultureMech_002798 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002798 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002798 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002798 --> ing10_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing11_NaCl["NaCl"]
    CultureMech_002798 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002798 --> ing12_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002798 --> ing13_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002798 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002798 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002798 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002798 --> ing17_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing17_AlK_SO4_2 -.-> CHEBI_86463
    ing18_H3BO3["H3BO3"]
    CultureMech_002798 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002798 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Biotin["Biotin"]
    CultureMech_002798 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_002798 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002798 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002798 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_002798 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002798 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002798 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_002798 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002798 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29_Lipoic_acid["Lipoic acid"]
    CultureMech_002798 --> ing29_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29_Lipoic_acid -.-> CHEBI_16494

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Sea Salt 3000 G_PER_L
2 Mg(NO3)2 x 6 H2O 254 G_PER_L
3 KH2PO4
H2O4P.K 136.084 g/mol
30 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NaHCO3
CHO3.Na 84.006 g/mol
100 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 MES 195 G_PER_L CHEBI:39010
MES
6 Sulfur
S 32.067 g/mol
1200 G_PER_L CHEBI:26833
sulfur atom
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
50 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.1 G_PER_L CHEBI:8806
Resazurin
9 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
12 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
13 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
14 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
15 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
18 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
19 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
22 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
23 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
26 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
28 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
29 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
30 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients except sulfur, NaHCO3 and Na2S x 9H2O, adjust pH to 6.5 and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml culture medium in 120 ml serum bottle containing 0.24 g sulfur) under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution and readjust pH to 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.644360Z mediadive-import Imported from MediaDive Source: JCM, ID: J448
2026-03-13T06:19:15.795839Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002798
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.237640+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KA22 MEDIUM" to "ka22_medium" for consistent matching
2026-06-05T07:41:10.308443+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.340662+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:39.496895+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint def64ed9fff71958f9ffe377961d7d12bafd14994826b299e0104f8383f627f7