Overview
- External ontology
- mediadive.medium:J448 (source link unavailable) — KA22 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=448
Composition
flowchart LR
CultureMech_002798["ka22_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_002798 --> ing0_Sea_Salt
ing1_Mg_NO3_2_x_6_H2O["Mg(NO3)2 x 6 H2O"]
CultureMech_002798 --> ing1_Mg_NO3_2_x_6_H2O
ing2_KH2PO4["KH2PO4"]
CultureMech_002798 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NaHCO3["NaHCO3"]
CultureMech_002798 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_MES["MES"]
CultureMech_002798 --> ing4_MES
CHEBI_39010["CHEBI:39010"]
ing4_MES -.-> CHEBI_39010
ing5_Sulfur["Sulfur"]
CultureMech_002798 --> ing5_Sulfur
CHEBI_26833["CHEBI:26833"]
ing5_Sulfur -.-> CHEBI_26833
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002798 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Resazurin["Resazurin"]
CultureMech_002798 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002798 --> ing8_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002798 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002798 --> ing10_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
ing11_NaCl["NaCl"]
CultureMech_002798 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002798 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002798 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002798 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002798 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002798 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002798 --> ing17_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing17_AlK_SO4_2 -.-> CHEBI_86463
ing18_H3BO3["H3BO3"]
CultureMech_002798 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002798 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_Biotin["Biotin"]
CultureMech_002798 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_002798 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002798 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002798 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_002798 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002798 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002798 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_002798 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002798 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_002798 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 3000 G_PER_L | ||
| 2 | Mg(NO3)2 x 6 H2O | 254 G_PER_L | ||
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
30 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
100 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 | MES | 195 G_PER_L |
CHEBI:39010
MES
|
|
| 6 |
Sulfur
S 32.067 g/mol
|
1200 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
50 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients except sulfur, NaHCO3 and Na2S x 9H2O, adjust pH to 6.5 and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Aseptically distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml culture medium in 120 ml serum bottle containing 0.24 g sulfur) under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution and readjust pH to 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.644360Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J448 |
| 2026-03-13T06:19:15.795839Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002798 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.237640+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KA22 MEDIUM" to "ka22_medium" for consistent matching |
| 2026-06-05T07:41:10.308443+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.340662+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |