Overview
- External ontology
- mediadive.medium:J514 (source link unavailable) — GLUCOSE SULFIDE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=514
Composition
flowchart LR
CultureMech_002865["glucose_sulfide_medium"]
ing0_Glucose["Glucose"]
CultureMech_002865 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_002865 --> ing1_Yeast_extract
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_002865 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_CaCO3["CaCO3"]
CultureMech_002865 --> ing3_CaCO3
CHEBI_3311["CHEBI:3311"]
ing3_CaCO3 -.-> CHEBI_3311
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_002865 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_KCl["KCl"]
CultureMech_002865 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_K2HPO4["K2HPO4"]
CultureMech_002865 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002865 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002865 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_Agar["Agar"]
CultureMech_002865 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Biotin["Biotin"]
CultureMech_002865 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_002865 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002865 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_002865 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Riboflavin["Riboflavin"]
CultureMech_002865 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_002865 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002865 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002865 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Myo_inositol["Myo-inositol"]
CultureMech_002865 --> ing18_Myo_inositol
CHEBI_17268["CHEBI:17268"]
ing18_Myo_inositol -.-> CHEBI_17268
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_002865 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
0.15 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.1 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.1 G_PER_L |
CHEBI:64205
calcium nitrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.05 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.001 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
Myo-inositol
C6H12O6 180.156 g/mol
|
0.001 G_PER_L |
CHEBI:17268
myo-inositol
|
|
| 20 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J514_GLUCOSE_SULFIDE_MEDIUM (CultureMech:002865)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.809533Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J514 |
| 2026-02-06T05:10:23.870162Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:16.187519Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002865 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.700320+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLUCOSE SULFIDE MEDIUM" to "glucose_sulfide_medium" for consistent matching |
| 2026-06-05T07:40:17.444176+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.359087+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:43.025518+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:36.924709+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 784130217dd6dd94b8407855e554c512f1c1606db193dfb2e5e4fc3b848b580b |