glucose_sulfide_medium

CultureMech:002865 bacterial COMPLEX SOLID_AGAR
Original name: GLUCOSE SULFIDE MEDIUM

Overview

External ontology
mediadive.medium:J514 (source link unavailable) — GLUCOSE SULFIDE MEDIUM
Applications
  • Microbial cultivation
pH
7.3
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=514

Composition

flowchart LR
    CultureMech_002865["glucose_sulfide_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_002865 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002865 --> ing1_Yeast_extract
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002865 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_CaCO3["CaCO3"]
    CultureMech_002865 --> ing3_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing3_CaCO3 -.-> CHEBI_3311
    ing4_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_002865 --> ing4_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing4_Ca_NO3_2 -.-> CHEBI_64205
    ing5_KCl["KCl"]
    CultureMech_002865 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002865 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002865 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002865 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_Agar["Agar"]
    CultureMech_002865 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_Biotin["Biotin"]
    CultureMech_002865 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_002865 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002865 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_002865 --> ing13_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing14_Riboflavin["Riboflavin"]
    CultureMech_002865 --> ing14_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_Riboflavin -.-> CHEBI_17015
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002865 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002865 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002865 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Myo_inositol["Myo-inositol"]
    CultureMech_002865 --> ing18_Myo_inositol
    CHEBI_17268["CHEBI:17268"]
    ing18_Myo_inositol -.-> CHEBI_17268
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_002865 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
0.15 G_PER_L CHEBI:17234
glucose
2 Yeast extract 1 G_PER_L
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 CaCO3
CO3.Ca 100.086 g/mol
0.1 G_PER_L CHEBI:3311
calcium carbonate
5 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.1 G_PER_L CHEBI:64205
calcium nitrate
6 KCl
Cl.K 74.551 g/mol
0.05 G_PER_L CHEBI:32588
potassium chloride
7 K2HPO4
HO4P.2K 174.174 g/mol
0.05 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 Agar 15 G_PER_L CHEBI:2509
agar
11 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
12 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
13 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
14 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.005 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
15 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
16 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
17 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.001 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Myo-inositol
C6H12O6 180.156 g/mol
0.001 G_PER_L CHEBI:17268
myo-inositol
20 Vitamin B12 1e-05 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.809533Z mediadive-import Imported from MediaDive Source: JCM, ID: J514
2026-02-06T05:10:23.870162Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:16.187519Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002865
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.700320+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GLUCOSE SULFIDE MEDIUM" to "glucose_sulfide_medium" for consistent matching
2026-06-05T07:40:17.444176+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.359087+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:43.025518+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.