a_mmx_medium

CultureMech:002938 bacterial COMPLEX LIQUID
Original name: A-MMX MEDIUM

Overview

External ontology
mediadive.medium:J590 (source link unavailable) — A-MMX MEDIUM
Applications
  • Microbial cultivation
pH
6.8
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=590

Composition

flowchart LR
    CultureMech_002938["a_mmx_medium"]
    ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002938 --> ing0_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing1_NaCl["NaCl"]
    CultureMech_002938 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002938 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002938 --> ing3_Yeast_extract
    ing4_Peptone["Peptone"]
    CultureMech_002938 --> ing4_Peptone
    ing5_Resazurin["Resazurin"]
    CultureMech_002938 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002938 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_Starch["Starch"]
    CultureMech_002938 --> ing7_Starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Starch -.-> CHEBI_28017
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002938 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002938 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_002938 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_NaBr["NaBr"]
    CultureMech_002938 --> ing11_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing11_NaBr -.-> CHEBI_63004
    ing12_H3BO3["H3BO3"]
    CultureMech_002938 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002938 --> ing13_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing14_KI["KI"]
    CultureMech_002938 --> ing14_KI
    CHEBI_8346["CHEBI:8346"]
    ing14_KI -.-> CHEBI_8346

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.69 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.19, 1.5]
2 NaCl
Cl.Na 58.443 g/mol
168.4 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 113.0, 55.4]
3 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 Yeast extract 0.2 G_PER_L
5 Peptone 0.2 G_PER_L
6 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 Starch 50 G_PER_L CHEBI:28017
starch
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
14 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
11 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 KCl
Cl.K 74.551 g/mol
1.3 G_PER_L CHEBI:32588
potassium chloride
12 NaBr
Br.Na 102.894 g/mol
0.2 G_PER_L CHEBI:63004
sodium bromide
13 H3BO3
H3BO3 61.833 g/mol
0.06 G_PER_L CHEBI:33118
boric acid
14 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
15 KI
I.K 166.002 g/mol
0.0001 G_PER_L CHEBI:8346
potassium iodide

Preparation

  1. Mix components except Na2S x 9H2O and bubble with N2 for 30 min. Distribute the medium into appropriate culture vessels (e.g., 10 ml medium in the Bellco anaerobic culture tube) under a N2 atmosphere, seal with butyl rubber stoppers. Add Na2S x 9H2O as 5% solution and autoclave. Adjust pH to 6.5 - 7.0, if necessary.
  2. Boil water, then, add starch and mix until dissolves thoroughly.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.020171Z mediadive-import Imported from MediaDive Source: JCM, ID: J590
2026-03-13T03:09:41.738704Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:16.685404Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002938
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.271485+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "A-MMX MEDIUM" to "a_mmx_medium" for consistent matching
2026-06-05T07:40:46.503270+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.314161+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.299511+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f79f35fdb3548dde79412455402f6a0e96e89adaafba1c62a8f4aa6d328accbc