Overview
- External ontology
- mediadive.medium:J590 (source link unavailable) — A-MMX MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=590
Composition
flowchart LR
CultureMech_002938["a_mmx_medium"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002938 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_NaCl["NaCl"]
CultureMech_002938 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KH2PO4["KH2PO4"]
CultureMech_002938 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Yeast_extract["Yeast extract"]
CultureMech_002938 --> ing3_Yeast_extract
ing4_Peptone["Peptone"]
CultureMech_002938 --> ing4_Peptone
ing5_Resazurin["Resazurin"]
CultureMech_002938 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002938 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Starch["Starch"]
CultureMech_002938 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002938 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002938 --> ing9_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
ing10_KCl["KCl"]
CultureMech_002938 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_NaBr["NaBr"]
CultureMech_002938 --> ing11_NaBr
CHEBI_63004["CHEBI:63004"]
ing11_NaBr -.-> CHEBI_63004
ing12_H3BO3["H3BO3"]
CultureMech_002938 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002938 --> ing13_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
ing14_KI["KI"]
CultureMech_002938 --> ing14_KI
CHEBI_8346["CHEBI:8346"]
ing14_KI -.-> CHEBI_8346
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.69 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.19, 1.5] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
168.4 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 113.0, 55.4] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 | Yeast extract | 0.2 G_PER_L | ||
| 5 | Peptone | 0.2 G_PER_L | ||
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 | Starch | 50 G_PER_L |
CHEBI:28017
starch
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
14 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.06 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 15 |
KI
I.K 166.002 g/mol
|
0.0001 G_PER_L |
CHEBI:8346
potassium iodide
|
Preparation
- Mix components except Na2S x 9H2O and bubble with N2 for 30 min. Distribute the medium into appropriate culture vessels (e.g., 10 ml medium in the Bellco anaerobic culture tube) under a N2 atmosphere, seal with butyl rubber stoppers. Add Na2S x 9H2O as 5% solution and autoclave. Adjust pH to 6.5 - 7.0, if necessary.
- Boil water, then, add starch and mix until dissolves thoroughly.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.020171Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J590 |
| 2026-03-13T03:09:41.738704Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:16.685404Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002938 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.271485+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A-MMX MEDIUM" to "a_mmx_medium" for consistent matching |
| 2026-06-05T07:40:46.503270+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.314161+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |