modified_mmjs_medium_b

Overview

External ontology

mediadive.medium:J592 (source link unavailable) — MODIFIED MMJS MEDIUM (B)

Applications

• Microbial cultivation

Synonyms

• {'name': 'mmmjs_pt', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J603', 'original_category': 'bacterial'}

pH

6.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=592

Composition

flowchart LR
    CultureMech_002940["modified_mmjs_medium_b"]
    ing0_NaCl["NaCl"]
    CultureMech_002940 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002940 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002940 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002940 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002940 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_NaNO3["NaNO3"]
    CultureMech_002940 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002940 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002940 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_002940 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002940 --> ing9_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002940 --> ing10_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing11_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_002940 --> ing11_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing11_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing12_H2WO4["H2WO4"]
    CultureMech_002940 --> ing12_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing12_H2WO4 -.-> CHEBI_36272
    ing13_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002940 --> ing13_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing13_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002940 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_Ferrous_citrate["Ferrous citrate"]
    CultureMech_002940 --> ing15_Ferrous_citrate
    ing16_NaHCO3["NaHCO3"]
    CultureMech_002940 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_Biotin["Biotin"]
    CultureMech_002940 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_002940 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002940 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002940 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Riboflavin["Riboflavin"]
    CultureMech_002940 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002940 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002940 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_002940 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002940 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing26_Lipoic_acid["Lipoic acid"]
    CultureMech_002940 --> ing26_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing26_Lipoic_acid -.-> CHEBI_16494

Ingredients (27)

Preparation

1. Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter):
2. Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture.
3. Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

• mmmjs_pt (CultureMech:002951)
• modified_mmjs_medium_b (CultureMech:002940)