Overview
- External ontology
- mediadive.medium:J592 (source link unavailable) — MODIFIED MMJS MEDIUM (B)
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=592
Composition
flowchart LR
CultureMech_002940["modified_mmjs_medium_b"]
ing0_NaCl["NaCl"]
CultureMech_002940 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_002940 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_002940 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002940 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_002940 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_NaNO3["NaNO3"]
CultureMech_002940 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002940 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002940 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_002940 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002940 --> ing9_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002940 --> ing10_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing11_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_002940 --> ing11_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing11_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing12_H2WO4["H2WO4"]
CultureMech_002940 --> ing12_H2WO4
CHEBI_36272["CHEBI:36272"]
ing12_H2WO4 -.-> CHEBI_36272
ing13_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002940 --> ing13_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing13_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002940 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_Ferrous_citrate["Ferrous citrate"]
CultureMech_002940 --> ing15_Ferrous_citrate
ing16_NaHCO3["NaHCO3"]
CultureMech_002940 --> ing16_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing16_NaHCO3 -.-> CHEBI_32139
ing17_Biotin["Biotin"]
CultureMech_002940 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_002940 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002940 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_002940 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_Riboflavin["Riboflavin"]
CultureMech_002940 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_002940 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002940 --> ing23_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_002940 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002940 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_Lipoic_acid["Lipoic acid"]
CultureMech_002940 --> ing26_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
19.4932 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.0877193 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0682261 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.779727 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.974659 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.974659 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.974659 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.84795 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.321637 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.000974659 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 11 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000974659 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 12 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
0.00974659 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 13 |
H2WO4
H2O4W 249.852 g/mol
|
0.000974659 G_PER_L |
CHEBI:36272
tungstic acid
|
|
| 14 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2.92398 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00974659 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 16 | Ferrous citrate | 0.00974659 G_PER_L | ||
| 17 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter):
- Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture.
- Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.025325Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J592 |
| 2026-03-13T06:19:16.698764Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002940 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.287489+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED MMJS MEDIUM (B)" to "modified_mmjs_medium_b" for consistent matching |
| 2026-06-05T07:41:37.392226+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.831015+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |