Overview
- External ontology
- mediadive.medium:J595 (source link unavailable) — CLOSTRIDIUM THERMOCELLUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=595
Composition
flowchart LR
CultureMech_002943["clostridium_thermocellum_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002943 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002943 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_002943 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_002943 --> ing3_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002943 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
CultureMech_002943 --> ing5_Sodium_beta_glycerophosphate
CHEBI_132089["CHEBI:132089"]
ing5_Sodium_beta_glycerophosphate -.-> CHEBI_132089
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002943 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Glutathione["Glutathione"]
CultureMech_002943 --> ing7_Glutathione
CHEBI_16856["CHEBI:16856"]
ing7_Glutathione -.-> CHEBI_16856
ing8_Yeast_extract["Yeast extract"]
CultureMech_002943 --> ing8_Yeast_extract
ing9_Resazurin["Resazurin"]
CultureMech_002943 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Cellulose["Cellulose"]
CultureMech_002943 --> ing10_Cellulose
CHEBI_18246["CHEBI:18246"]
ing10_Cellulose -.-> CHEBI_18246
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.43 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
7.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
|
6 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0011 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
Glutathione
C10H17N3O6S 307.328 g/mol
|
0.25 G_PER_L |
CHEBI:16856
glutathione
|
|
| 9 | Yeast extract | 4.5 G_PER_L | ||
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
10 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Preparation
- Adjust pH to 7.0 - 7.2.
- Gas atmosphere: 95% N2 + 5% CO2.
- Comment: Cellulose can be replaced with 5.0 g/L cellobiose. In this case, cellobiose is filter-sterilized separately as 10% (w/v) solution in distilled water.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- clostridium_thermocellum_medium (CultureMech:002943)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.038635Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J595 |
| 2026-03-13T06:19:16.728338Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002943 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.322834+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM THERMOCELLUM MEDIUM" to "clostridium_thermocellum_medium" for consistent matching |
| 2026-06-05T07:40:54.327967+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.850217+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.677630+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f6f3f2369baf3d5f0ba5e4c2554000c7ab0615490920475c17df87b2c27e2460 |